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Journal articles
sgDI-tector: defective interfering viral genome bioinformatics for detection of coronavirus subgenomic RNAs
Abstract : Coronavirus RNA-dependent RNA polymerases produce subgenomic RNAs (sgRNAs) that encode viral structural and accessory proteins. User-friendly bioinformatic tools to detect and quantify sgRNA production are urgently needed to study the growing number of next-generation sequencing (NGS) data of SARS-CoV-2. We introduced sgDI-tector to identify and quantify sgRNA in SARS-CoV-2 NGS data. sgDI-tector allowed detection of sgRNA without initial knowledge of the transcription-regulatory sequences. We produced NGS data and successfully detected the nested set of sgRNAs with the ranking M > ORF3a > N>ORF6 > ORF7a > ORF8 > S > E>ORF7b. We also compared the level of sgRNA production with other types of viral RNA products such as defective interfering viral genomes.
https://hal-pasteur.archives-ouvertes.fr/pasteur-03591131
Contributor : Rachel Legendre Connect in order to contact the contributor
Submitted on : Monday, February 28, 2022 - 1:51:24 PM
Last modification on : Tuesday, April 19, 2022 - 2:58:38 PM
Contributor : Rachel Legendre Connect in order to contact the contributor
Submitted on : Monday, February 28, 2022 - 1:51:24 PM
Last modification on : Tuesday, April 19, 2022 - 2:58:38 PM
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RNA-2022-Di Gioacchino-277-89....
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