Specificity of mouse and human Fcgamma receptors and their polymorphic variants for IgG subclasses of different species

Yu Wang (1, 2) , Vanessa Krémer (1, 2) , Bruno Iannascoli (1, 2) , Odile Richard Le Goff (1, 2) , David A Mancardi (1, 2) , Leoni Ramke (1, 2) , Luc de Chaisemartin (1, 2, 3) , Pierre Bruhns (1, 2) , Friederike Jönsson (1, 4, 2)

1 Anticorps en thérapie et pathologie - Antibodies in Therapy and Pathology
2 UPCité - Université Paris Cité
3 AP-HP - Hôpital Bichat - Claude Bernard [Paris]
4 CNRS - Centre National de la Recherche Scientifique

Immunoglobulin G (IgG) is the predominant antibody class generated during infections and used for the generation of therapeutic antibodies. Antibodies are mainly characterized in or generated from animal models that support particular infections, respond to particular antigens or allow the generation of hybridomas. Due to the availability of numerous transgenic mouse models and the ease of performing bioassays with human blood cells in vitro, most antibodies from species other than mice and humans are tested in vitro using human cells and/or in vivo using mice. In this process, it is expected, but not yet systematically documented, that IgG from these species interact with human or mouse IgG receptors (FcRs). In this study, we undertook a systematic assessment of binding specificities of IgG from various species to the families of mouse and human FcRs, including their polymorphic variants. Our results document the specific binding patterns for each of these IgG (sub)classes, reveal possible caveats of antibody-based immunoassays, and will be a useful reference for the transition from one animal model to preclinical mouse models or human cell-based bioassays.

Keywords: IgG; Fcγ receptors; cross-binding; immune complexes

Domains: Immunology

Yu Wang, Vanessa Krémer, Bruno Iannascoli, Odile Richard Le Goff, David A Mancardi, et al.. Specificity of mouse and human Fcgamma receptors and their polymorphic variants for IgG subclasses of different species. European Journal of Immunology, 2022, ⟨10.1002/eji.202149766⟩. ⟨pasteur-03566563⟩