HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Insights into the ancestry evolution of the Mycobacterium tuberculosis complex from analysis of Mycobacterium riyadhense

Qingtian Guan 1 Musa Garbati 2 Sara Mfarrej 1 Talal Almutairi 2 Thomas Laval 3, 4 Albel Singh 5 Shamsudeen Fagbo 6, 7 Alicia Smyth 8 John Browne 8 Muhammad amin Urrahman 2 Alya Alruwaili 2 Anwar Hoosen 2 Conor Meehan 9 Chie Nakajima 10 Yasuhiko Suzuki 10 Caroline Demangel 3 Apoorva Bhatt 5 Stephen Gordon 8, 10 Faisal Alasmari 2 Arnab Pain 1, 10, *
* Corresponding author
1 KAUST - King Abdullah University of Science and Technology
2 KFMC - King Fahad Medical City [Riyadh]
3 Immunobiologie de l'Infection - Immunobiology of Infection
4 UPD7 - Université Paris Diderot - Paris 7
5 University of Birmingham [Birmingham]
6 Saudi Center for Disease Prevention and Control [Riyadh, Saudi Arabia]
7 NIMR - Nigerian Institute of Medical Research [Yaba, Lagos, Nigeria]
8 UCD - University College Dublin [Dublin]
9 University of Bradford
10 Hokkaido University [Sapporo, Japan]
Abstract : Current evolutionary scenarios posit the emergence of Mycobacterium tuberculosis from an environmental saprophyte through a cumulative process of genome adaptation. Mycobacterium riyadhense, a related bacillus, is being increasingly isolated from human clinical cases with tuberculosis-like symptoms in various parts of the world. To elucidate the evolutionary relationship between M. riyadhense and other mycobacterial species, including members of the M. tuberculosis complex (MTBC), eight clinical isolates of M. riyadhense were sequenced and analyzed. We show, among other features, that M. riyadhense shares a large number of conserved orthologs with M. tuberculosis and shows the expansion of toxin/antitoxin pairs, PE/PPE family proteins compared with other non-tuberculous mycobacteria. We observed M. riyadhense lacks wecE gene which may result in the absence of lipooligosaccharides (LOS) IV. Comparative transcriptomic analysis of infected macrophages reveals genes encoding inducers of Type I IFN responses, such as cytosolic DNA sensors, were relatively less expressed by macrophages infected with M. riyadhense or M. kansasii, compared to BCG or M. tuberculosis. Overall, our work sheds new light on the evolution of M. riyadhense, its relationship to the MTBC, and its potential as a system for the study of mycobacterial virulence and pathogenesis.
Document type :
Journal articles
Complete list of metadata
https://hal-pasteur.archives-ouvertes.fr/pasteur-03534725
Contributor : Marie-Luce Kop Connect in order to contact the contributor
Submitted on : Wednesday, January 19, 2022 - 3:28:04 PM
Last modification on : Tuesday, April 19, 2022 - 11:46:01 AM
Long-term archiving on: : Wednesday, April 20, 2022 - 6:57:32 PM

File

Vignette du document
lqab070.pdf
Publication funded by an institution

Licence

Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

PASTEUR | INSERM | UNIV-PARIS

Citation

Qingtian Guan, Musa Garbati, Sara Mfarrej, Talal Almutairi, Thomas Laval, et al.. Insights into the ancestry evolution of the Mycobacterium tuberculosis complex from analysis of Mycobacterium riyadhense. NAR Genomics and Bioinformatics, Oxford University Press, 2021, 3 (3), pp.lqab070. ⟨10.1093/nargab/lqab070⟩. ⟨pasteur-03534725⟩

Export

BibTeX TEI DC DCterms EndNote Datacite

Share

Metrics

Record views

22

Files downloads

6