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Journal articles
Several regions of the repeat domain of the Staphylococcus caprae autolysin, AtlC, are involved in fibronectin binding
Abstract : The autolysin AtlC is the only known fibronectin-binding protein in Staphylococcus caprae strain 96007. The fibronectin-binding domain of AtlC consists of three repeats (AtlCR 1 R 2 R 3), which are located between the two enzymatic domains. The AtlCR 1 R 2 R 3 domain and the AtlCR 1 R 2 and AtlCR 3 subdomains were expressed separately as His 6-tagged proteins. In Western affinity blots, only AtlCR 1 R 2 R 3 and AtlCR 3 but not AtlCR 1 R 2 appeared to recognise fibronectin; however, in ELISA and Biacore experiments, all three bound fibronectin. The interaction between AtlCR 1 R 2 R 3 and fibronectin is multivalent and involves high-and low-affinity sites that are present in a 2:1 ratio. These distinct classes of binding sites may be situated on either or on both ligands.
https://hal-pasteur.archives-ouvertes.fr/pasteur-03525667
Contributor : Patrick England Connect in order to contact the contributor
Submitted on : Friday, January 14, 2022 - 6:20:05 AM
Last modification on : Thursday, April 7, 2022 - 10:10:55 AM
Contributor : Patrick England Connect in order to contact the contributor
Submitted on : Friday, January 14, 2022 - 6:20:05 AM
Last modification on : Thursday, April 7, 2022 - 10:10:55 AM
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