HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

The α-helical regions of KERP1 are important in Entamoeba histolytica adherence to human cells

Abstract : The lysine and glutamic acid rich protein KERP1 is a unique surface adhesion factor associated with virulence in the human pathogen Entamoeba histolytica. Both the function and structure of this protein remain unknown to this date. Here, we used circular dichroism, analytical ultracentrifugation and bioinformatics modeling to characterize the structure of KERP1. Our findings revealed that it is an α-helical rich protein organized as a trimer, endowed with a very high thermal stability (Tm = 89.6°C). Bioinformatics sequence analyses and 3D-structural modeling indicates that KERP1 central segments could account for protein trimerization. Relevantly, expressing the central region of KERP1 in living parasites, impair their capacity to adhere to human cells. Our observations suggest a link between the inhibitory effect of the isolated central region and the structural features of KERP1.
Document type :
Journal articles
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03525604
Contributor : Patrick England Connect in order to contact the contributor
Submitted on : Thursday, January 13, 2022 - 11:17:41 PM
Last modification on : Thursday, April 7, 2022 - 10:10:53 AM
Long-term archiving on: : Friday, April 15, 2022 - 2:04:21 AM

File

Perdomo et al KERP1 Sci Rep ja...
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution - NonCommercial - ShareAlike 4.0 International License

Identifiers

Citation

Doranda Perdomo, Bruno Baron, Arturo Rojo-Domínguez, Bertrand Raynal, Patrick England, et al.. The α-helical regions of KERP1 are important in Entamoeba histolytica adherence to human cells. Scientific Reports, Nature Publishing Group, 2013, 3 (1), pp.1171. ⟨10.1038/srep01171⟩. ⟨pasteur-03525604⟩

Share

Metrics

Record views

8

Files downloads

25