Signature-Tagged Transposon Mutagenesis Identifies Novel Mycobacterium tuberculosis Genes Involved in the Parasitism of Human Macrophages

Vania Rosas-Magallanes (1) , Gustavo Stadthagen-Gomez (1) , Jean Rauzier (1) , Luis Barreiro (1) , Ludovic Tailleux (1) , Frédéric Boudou (1) , Ruth Griffin (2) , Jérome Nigou (3) , Mary Jackson (1) , Brigitte Gicquel (1) , Olivier Neyrolles (1)

1 Génétique mycobactérienne - Mycobacterial genetics
2 Imperial College London
3 IPBS - Institut de pharmacologie et de biologie structurale

Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.

Domains: Innate immunity; Infectious diseases

Vania Rosas-Magallanes, Gustavo Stadthagen-Gomez, Jean Rauzier, Luis Barreiro, Ludovic Tailleux, et al.. Signature-Tagged Transposon Mutagenesis Identifies Novel Mycobacterium tuberculosis Genes Involved in the Parasitism of Human Macrophages. Infection and Immunity, 2007, 75 (1), pp.504-507. ⟨10.1128/IAI.00058-06⟩. ⟨pasteur-03525490⟩