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Efficient monitoring of protein ubiquitylation levels using TUBEs-based microarrays

Abstract : Analyzing protein ubiquitylation changes during physiological or pathological processes is challenging due to its high reversibility and dynamic turnover of modified targets. We have developed a protein microarray to assess endogenous ubiquitylation levels from cell cultures, employing tandem ubiquitin-binding entities (TUBEs) with three or four ubiquitin-associated (UBA) domains as capture probes. Adriamycin (ADR)-stimulated MCF7 cells were used to differentiate protein ubiquitylation levels between cells that are sensitive or resistant to ADR treatment. We show that TUBEs-based microarrays can be used for the analysis of cellular processes regulated by ubiquitylation and for the detection of pathologies with aberrant ubiquitylation levels.
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Submitted on : Thursday, January 13, 2022 - 7:26:19 PM
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Sonia Serna, Wendy Xolalpa, Valérie Lang, Fabienne Aillet, Patrick England, et al.. Efficient monitoring of protein ubiquitylation levels using TUBEs-based microarrays. FEBS Letters, 2016, 590 (16), pp.2748 - 2756. ⟨10.1002/1873-3468.12289⟩. ⟨pasteur-03525440⟩



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