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Journal articles
Manganese cations increase the mutation rate of human immunodeficiency virus type 1 ex vivo
Abstract : Human immunodeficiency virus (HIV) reverse transcription is an error-prone process with an overall mutation rate of ∼3·4×10 −5 per base per replication cycle. This rate can be modulated by changes in different components of the retrotranscription reaction. In particular, in vitro substitution of magnesium cations (Mg 2+ ) by manganese cations (Mn 2+ ) has been shown to increase misincorporation of deoxynucleotide triphosphates (dNTPs) and to alter substrate specificity. Here, it is shown that Mn 2+ also increases the HIV mutation rate ex vivo . Treatment of permissive cells with Mn 2+ and subsequent HIV infection resulted in at least 6-fold and 10-fold increases in the mutant and mutation frequencies respectively, thus illustrating a further example of how to influence HIV genetic variation.
https://hal-pasteur.archives-ouvertes.fr/pasteur-03520172
Contributor : Jean-Pierre Vartanian Connect in order to contact the contributor
Submitted on : Monday, January 10, 2022 - 7:46:20 PM
Last modification on : Thursday, January 13, 2022 - 6:13:40 PM
Contributor : Jean-Pierre Vartanian Connect in order to contact the contributor
Submitted on : Monday, January 10, 2022 - 7:46:20 PM
Last modification on : Thursday, January 13, 2022 - 6:13:40 PM
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