Srand specific PCR amplification of low copy number DNA

Andreas Meyerhans (1) , Jean Pierre Vartanian (1) , Simon Wain-Hobson (1)

1 Rétrovirologie Moléculaire

A method for the amplification of a single DNA strand at low copy number is described. It is a wholly PCR based approach which involves an initial linear amplification of the target using a tagged strand specific primer. This is followed by classical PCR amplification of the progeny using a pair of primers, one specific for the sequence tagged onto the 5' end of the first round primer, the second specific for the target sequence. Given the protocol used the ratio of the two strands in the final amplification product was 50:1.

Domaines: Sciences du Vivant [q-bio]

Andreas Meyerhans, Jean Pierre Vartanian, Simon Wain-Hobson. Srand specific PCR amplification of low copy number DNA. Nucleic Acids Research, 1992, 20 (3), pp.521-523. ⟨10.1093/nar/20.3.521⟩. ⟨pasteur-03520147⟩