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DNA recombination during PCR

Abstract : PCR co-amplification of two distinct HIV1 tat gene sequences lead to the formation of recombinant DNA molecules. The frequency of such recombinants, up to 5.4% of all amplified molecules, could be decreased 2.7 fold by a 6 fold increase in Taq DNA polymerase elongation time. Crossover sites mapped essentially to three discrete regions suggesting specific Taq DNA polymerase pause or termination sites. PCR mediated recombination may be a problem when studying heterogeneous genetic material such as RNA viruses, multigene families, or repetitive sequences. This phenomenon can be exploited to create chimeric molecules from related sequences.
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Contributor : Jean-Pierre Vartanian Connect in order to contact the contributor
Submitted on : Monday, January 10, 2022 - 7:14:57 PM
Last modification on : Thursday, April 7, 2022 - 10:10:55 AM

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Andreas Meyerhans, Jean Pierre Vartanian, Simon Wain-Hobson. DNA recombination during PCR. Nucleic Acids Research, 1990, 18 (7), pp.1687-1691. ⟨10.1093/nar/18.7.1687⟩. ⟨pasteur-03520139⟩



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