Intravital Two-Photon Imaging of Natural Killer Cells and Dendritic Cells in Lymph Nodes

Susanna Celli (1) , Béatrice Breart (1) , Philippe Bousso (1)

1 Dynamiques des Réponses Immunes

Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of natural killer (NK) cells and dendritic cells (DC) in the lymph nodes of live, anesthetized mice. Intravital two-photon imaging is a powerful tool to study the migration and cell interactions of immune cells such as NK and DC in physiological settings.

Mots clés: NK cells; dendritic cells; lymph node; intravital imaging; two-photon microscopy

Domaines: Immunologie

Susanna Celli, Béatrice Breart, Philippe Bousso. Intravital Two-Photon Imaging of Natural Killer Cells and Dendritic Cells in Lymph Nodes. Jonathan Ewbank; Eric Vivier. Innate Immunity, 415, Humana Press, pp.119-126, 2008, 978-1-59745-570-1 (ebook). ⟨10.1007/978-1-59745-570-1_7⟩. ⟨pasteur-03517225⟩