Global Run-On sequencing to measure nascent transcription in C. elegans

Piergiuseppe Quarato (1) , Germano Cecere (1)

1 Mécanismes de l'Hérédité épigénétique / Mechanisms of epigenetic inheritance

Global Run-On sequencing (GRO-seq) is one of the most sensitive techniques to detect nascent transcription from RNA polymerase (Pol) at a genome-wide level. The protocol incorporates labeled ribonucleotides into nascent RNAs from Pol I, II, and III. We have adapted the GRO-seq protocol to the nematode Caenorhabditis elegans to measure transcription from embryos and adult worms. Here, we provide a detailed overview of the protocol highlighting the critical steps for generating successful libraries. For complete details on the use and execution of this protocol, please refer to Quarato et al. (2021).

Keywords: Cell separation/fractionation; Gene Expression; Genomics; Model Organisms; Molecular Biology; RNAseq; Sequencing

Domains: Life Sciences [q-bio]

Piergiuseppe Quarato, Germano Cecere. Global Run-On sequencing to measure nascent transcription in C. elegans. STAR Protocols, 2021, 2 (4), pp.100991. ⟨10.1016/j.xpro.2021.100991⟩. ⟨pasteur-03514388⟩