CD4 T cells integrate signals delivered during successive DC encounters in vivo

Susanna Celli (1) , Zacarias Garcia (1) , Philippe Bousso (1)

1 Dynamiques des Réponses Immunes

The cellular mode of T cell priming in vivo remains to be characterized fully. We investigated the fate of T cell-dendritic cell (DC) interactions in the late phase of T cell activation in the lymph node. In general, CD4 T cells detach from DCs before undergoing cell division. Using a new approach to track the history of antigen (Ag)-recognition events, we demonstrated that activated/divided T cells reengage different DCs in an Ag-specific manner. Two-photon imaging of intact lymph nodes suggested that T cells could establish prolonged interactions with DCs at multiple stages during the activation process. Importantly, signals that are delivered during subsequent DC contacts are integrated by the T cell and promote sustained IL-2R ␣ expression and IFN-␥ production. Thus, repeated encounters with Ag-bearing DCs can occur in vivo and modulate CD4 T cell differentiation programs.

Mots clés: Ag; antigen; CFSE; carboxyl fluorescein succinimidyl ester; i.d.; intradermally

Domaines: Immunologie

Susanna Celli, Zacarias Garcia, Philippe Bousso. CD4 T cells integrate signals delivered during successive DC encounters in vivo. Journal of Experimental Medicine, 2005, 202 (9), pp.1271 - 1278. ⟨10.1084/jem.20051018⟩. ⟨pasteur-03513333⟩