High prevalence of intestinal parasite infestations among stunted and control children aged 2 to 5 years old in two neighborhoods of Antananarivo, Madagascar

Azimdine Habib (1) , Lova Andrianonimiadana (1) , Maheninasy Rakotondrainipiana (2) , Prisca Andriantsalama (2) , Ravaka Randriamparany (2) , Rindra Vatosoa Randremanana (2) , Rado Rakotoarison (3) , Inès Vigan‑womas (3) , Armand Rafalimanantsoa (4) , Pascale Vonaesch (5) , Philippe J. Sansonetti (5) , Jean-Marc Collard (1) , The Afribiota Investigators

1 IPM - Unité de Bactériologie Expérimentale [Antananarivo, Madagascar]
2 Unité d’Épidémiologie et de Recherche clinique [Antananarivo, Madagascar]
3 IPM - Unité d'immunologie des maladies infectieuses [Antananarivo, Madagascar]
4 IPM - Unité des Helminthiases [Antananarivo, Madagascar]
5 Pathogénie microbienne moléculaire

Background This study aimed to compare the prevalence of intestinal parasite infestations (IPIs) in stunted children, compared to control children, in Ankasina and Andranomanalina Isotry (two disadvantaged neighborhoods of Antananarivo, Madagascar), to characterize associated risk factors and to compare IPI detection by real-time PCR and standard microscopy techniques. Methodology/Principal findings Fecal samples were collected from a total of 410 children (171 stunted and 239 control) aged 2–5 years. A single stool sample per subject was examined by simple merthiolate-iodine-formaldehyde (MIF), Kato-Katz smear and real-time PCR techniques. A total of 96.3% of the children were infested with at least one intestinal parasite. The most prevalent parasites were Giardia intestinalis (79.5%), Ascaris lumbricoides (68.3%) and Trichuris trichiura (68.0%). For all parasites studied, real-time PCR showed higher detection rates compared to microscopy ( G . intestinalis [77.6% (n = 318) versus 20.9% (n = 86)], Entamoeba histolytica [15.8% (n = 65) versus 1.9% (n = 8)] and A . lumbricoides [64.1% (n = 263) versus 50.7% (n = 208)]). Among the different variables assessed in the study, age of 4 to 5 years (AOR = 4.61; 95% CI, (1.35–15.77)) and primary and secondary educational level of the mother (AOR = 12.59; 95% CI, (2.76–57.47); AOR = 9.17; 95% CI, (2.12–39.71), respectively) were significantly associated with IPIs. Children drinking untreated water was associated with infestation with G . intestinalis (AOR = 1.85; 95% CI, (1.1–3.09)) and E . histolytica (AOR = 1.9; 95% CI, (1.07–3.38)). E . histolytica was also associated with moderately stunted children (AOR = 0.37; 95% CI, 0.2–0.71). Similarly, children aged between 4 and 5 years (AOR = 3.2; 95% CI (2.04–5.01)) and living on noncemented soil types (AOR = 1.85; 95% CI, (1.18–2.09)) were associated with T . trichiura infestation. Conclusions/Significance The prevalence of IPIs is substantial in the studied areas in both stunted and control children, despite the large-scale drug administration of antiparasitic drugs in the country. This high prevalence of IPIs warrants further investigation. Improved health education, environmental sanitation and quality of water sources should be provided.

Domaines: Microbiologie et Parasitologie; Immunologie; Biologie cellulaire; Biologie moléculaire; Santé publique et épidémiologie

Azimdine Habib, Lova Andrianonimiadana, Maheninasy Rakotondrainipiana, Prisca Andriantsalama, Ravaka Randriamparany, et al.. High prevalence of intestinal parasite infestations among stunted and control children aged 2 to 5 years old in two neighborhoods of Antananarivo, Madagascar. PLoS Neglected Tropical Diseases, 2021, 15 (4), pp.e0009333. ⟨10.1371/journal.pntd.0009333⟩. ⟨pasteur-03506537⟩