Dichotomous metabolic networks govern human ILC2 proliferation and function - Institut Pasteur Access content directly
Journal Articles Nature Immunology Year : 2021

Dichotomous metabolic networks govern human ILC2 proliferation and function

Laura Surace
Jean-Marc Doisne
Ido Amit


Group 2 innate lymphoid cells (ILC2s) represent innate homologs of type 2 helper T cells (T H 2) that participate in immune defense and tissue homeostasis through production of type 2 cytokines. While T lymphocytes metabolically adapt to microenvironmental changes, knowledge of human ILC2 metabolism is limited, and its key regulators are unknown. Here, we show that circulating ‘naive’ ILC2s have an unexpected metabolic profile with a higher level of oxidative phosphorylation (OXPHOS) than natural killer (NK) cells. Accordingly, ILC2s are severely reduced in individuals with mitochondrial disease (MD) and impaired OXPHOS. Metabolomic and nutrient receptor analysis revealed ILC2 uptake of amino acids to sustain OXPHOS at steady state. Following activation with interleukin-33 (IL-33), ILC2s became highly proliferative, relying on glycolysis and mammalian target of rapamycin (mTOR) to produce IL-13 while continuing to fuel OXPHOS with amino acids to maintain cellular fitness and proliferation. Our results suggest that proliferation and function are metabolically uncoupled in human ILC2s, offering new strategies to target ILC2s in disease settings.


Fichier principal
Vignette du fichier
2021_Nat Immunol_Surace.pdf (7.15 Mo) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

pasteur-03499188 , version 1 (21-12-2021)





Laura Surace, Jean-Marc Doisne, Carys A Croft, Anna Thaller, Pedro Escoll, et al.. Dichotomous metabolic networks govern human ILC2 proliferation and function. Nature Immunology, 2021, 22 (11), pp.1367-1374. ⟨10.1038/s41590-021-01043-8⟩. ⟨pasteur-03499188⟩
34 View
45 Download



Gmail Facebook Twitter LinkedIn More