 |
Journal articles
mRNA vaccination of naive and COVID-19-recovered individuals elicits potent memory B cells that recognize SARS-CoV-2 variants
Aurélien Sokal
1, 2
Giovanna Barba-Spaeth
3
Ignacio Fernández
3
Matteo Broketa
4, 5
Imane Azzaoui
2, 6
Andréa de la Selle
1, 2
Alexis Vandenberghe
2, 6
Slim Fourati
7, 6
Anais Roeser
1, 2
Annalisa Meola
3
Magali Bouvier-Alias
7, 6
Etienne Crickx
1, 2
Laetitia Languille
2
Marc Michel
2
Bertrand Godeau
2
Sébastien Gallien
8, 9
Giovanna Melica
8, 9
Yann Nguyen
10
Virginie Zarrouk
10
Florence Canoui-Poitrine
11
France Pirenne
6, 12
Jérôme Mégret
13
Jean-Michel Pawlotsky
7, 6
Simon Fillatreau
1
Pierre Bruhns
4
Felix Rey
3
Jean-Claude Weill
1, *
Claude-Agnès Reynaud
1, *
Pascal Chappert
1, 14, *
Matthieu Mahévas
1, 2, 6, *
*
Corresponding author
14
Inovarion
Aurélien Sokal 1, 2
Author
Giovanna Barba-Spaeth 3
Author IdHAL : giovanna-barba-spaeth ORCID : https://orcid.org/0000-0003-2069-9501
Ignacio Fernández 3
Author
Matteo Broketa 4, 5
Author ORCID : https://orcid.org/0000-0001-7825-1174
Imane Azzaoui 2, 6
Author ORCID : https://orcid.org/0000-0001-5603-6780
Andréa de la Selle 1, 2
Author
Alexis Vandenberghe 2, 6
Author
Slim Fourati 7, 6
Author
Anais Roeser 1, 2
Author ORCID : https://orcid.org/0000-0001-6717-3115
Annalisa Meola 3
Author
Magali Bouvier-Alias 7, 6
Author ORCID : https://orcid.org/0000-0003-1590-5980
Etienne Crickx 1, 2
Author
Laetitia Languille 2
Author
Marc Michel 2
Author
Bertrand Godeau 2
Author
Sébastien Gallien 8, 9
Author
Giovanna Melica 8, 9
Author
Yann Nguyen 10
Author
Virginie Zarrouk 10
Author
Florence Canoui-Poitrine 11
Author
France Pirenne 6, 12
Author
Jérôme Mégret 13
Author
Jean-Michel Pawlotsky 7, 6
Author ORCID : https://orcid.org/0000-0003-0745-7559
Simon Fillatreau 1
Author
Pierre Bruhns 4
Author IdHAL : pierre-bruhns ResearcherId : http://www.researcherid.com/rid/F-5567-2013 ORCID : https://orcid.org/0000-0002-4709-8936
Felix A. Rey 3
Author ORCID : https://orcid.org/0000-0002-9953-7988
Jean-Claude Weill 1
Correspondent author
Claude-Agnès Reynaud 1
Correspondent author
Pascal Chappert 1, 14
Correspondent author ORCID : https://orcid.org/0000-0002-9799-4608
Matthieu Mahévas 1, 2, 6
Correspondent author
1
INEM - UM 111 (UMR 8253 / U1151) - Institut Necker Enfants-Malades (Bâtiment Leriche
14 rue Maria Helena Vieira Da Silva
75014 Paris - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1151 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 8253 (France)
- UP - Université de Paris : UM 111 (85 boulevard Saint-Germain 75006 Paris - France)
2
Service de médecine interne [Mondor] (51 Av du Maréchal de Lattre de Tassigny, 94000 Créteil - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- Hôpital Henri Mondor (France)
- UPEC UP12 - Université Paris-Est Créteil Val-de-Marne - Paris 12 (61 avenue du Général de Gaulle - 94010 Créteil cedex - France)
3
Virologie Structurale - Structural Virology (Département de Virologie - 25-28, rue du docteur Roux 75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR3569 (France)
4
Anticorps en thérapie et pathologie - Antibodies in Therapy and Pathology (Département d'Immunologie, 25-28, rue du Docteur Roux, 75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1222 (101, rue de Tolbiac, 75013 Paris - France)
6
IMRB - Institut Mondor de Recherche Biomédicale (8 rue du Général Sarrail 94010 Créteil - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U955 (101, rue de Tolbiac, 75013 Paris - France)
- UPEC UP12 - Université Paris-Est Créteil Val-de-Marne - Paris 12 : UMR955 (61 avenue du Général de Gaulle - 94010 Créteil cedex - France)
- IFR10 (France)
7
Service de bactériologie, virologie, hygiène [Mondor] (51 Av Maréchal de Lattre de Tassigny, 94000 Créteil - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- Hôpital Henri Mondor (France)
- UPEC UP12 - Université Paris-Est Créteil Val-de-Marne - Paris 12 (61 avenue du Général de Gaulle - 94010 Créteil cedex - France)
8
Hôpital Henri Mondor (France)
9
UPEC UP12 - Université Paris-Est Créteil Val-de-Marne - Paris 12 (61 avenue du Général de Gaulle - 94010 Créteil cedex - France)
10
Hôpital Beaujon [AP-HP] (100, boulevard du Général Leclerc 92 Clichy - France)
11
CEpiA - Clinical Epidemiology and Ageing : Geriatrie Soins Primaires et Santé Publique (Service de santé publique
CHU Henri Mondor
51 Avenue du Maréchal de Lattre de Tassigny
94010 Créteil Cedex
- France)
- UPEC UP12 - Université Paris-Est Créteil Val-de-Marne - Paris 12 : EA7376 (61 avenue du Général de Gaulle - 94010 Créteil cedex - France)
12
Etablissement Français du Sang (France)
13
SFR Necker - UMS 3633 / US24 - Structure Fédérative de Recherche Necker (Faculté Paris Descartes - Bâtiment Leriche Porte 14 -
14 rue Maria Helena Vieira Da Silva - CS 61431
75993 Paris cedex 14 - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : US24 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMS3633 (France)
- UP - Université de Paris : UMS 3633 (85 boulevard Saint-Germain 75006 Paris - France)
14
Inovarion (Tour Onyx, 10 rue Vandrezanne, 75013 Paris - France)
Abstract : In addition to serum immunoglobulins, memory B cell (MBC) generation against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is another layer of immune protection, but the quality of MBC responses in naive and coronavirus disease 2019 (COVID-19)-recovered individuals after vaccination remains ill defined. We studied longitudinal cohorts of naive and disease-recovered individuals for up to 2 months after SARS-CoV-2 mRNA vaccination. We assessed the quality of the memory response by analysis of antibody repertoires, affinity, and neutralization against variants of concern (VOCs) using unbiased cultures of 2,452 MBCs. Upon boosting, the MBC pool of recovered individuals expanded selectively, matured further, and harbored potent neutralizers against VOCs. Although naive individuals had weaker neutralizing serum responses, half of their RBD-specific MBCs displayed high affinity toward multiple VOCs, including delta (B.1.617.2), and one-third retained neutralizing potency against beta (B.1.351). Our data suggest that an additional challenge in naive vaccinees could recall such affinity-matured MBCs and allow them to respond efficiently to VOCs.
Document type :
Journal articles
Complete list of metadata
https://hal-pasteur.archives-ouvertes.fr/pasteur-03423964
Contributor : Giovanna Barba Spaeth Connect in order to contact the contributor
Submitted on : Wednesday, November 10, 2021 - 2:34:14 PM
Last modification on : Tuesday, November 16, 2021 - 5:25:20 AM
Contributor : Giovanna Barba Spaeth Connect in order to contact the contributor
Submitted on : Wednesday, November 10, 2021 - 2:34:14 PM
Last modification on : Tuesday, November 16, 2021 - 5:25:20 AM
File
ViewPageProof_IMMUNI_4715 (2)....
Files produced by the author(s)
Identifiers
- HAL Id : pasteur-03423964, version 1
- DOI : 10.1016/j.immuni.2021.09.011
- PUBMED : 34614412
- PUBMEDCENTRAL : PMC8452492
Collections
PASTEUR | IMRB | UPEC-UPEM | UPEC | UP-SANTE | ANR | CNRS | UNIV-PARIS | SORBONNE-UNIVERSITE | SU-TI
Citation
Aurélien Sokal, Giovanna Barba-Spaeth, Ignacio Fernández, Matteo Broketa, Imane Azzaoui, et al.. mRNA vaccination of naive and COVID-19-recovered individuals elicits potent memory B cells that recognize SARS-CoV-2 variants. Immunity, Elsevier, 2021, ⟨10.1016/j.immuni.2021.09.011⟩. ⟨pasteur-03423964⟩
Metrics
Record views
37
Files downloads