Generation of gene-level resolution chromosome contact maps in bacteria and archaea

Charlotte Cockram (1) , Agnès Thierry (1) , Romain Koszul (1)

1 Régulation spatiale des Génomes - Spatial Regulation of Genomes

Chromosome conformation capture (Hi-C) has become a routine method for probing the 3D organization of genomes. However, when applied to bacteria and archaea, current protocols are expensive and limited in their resolution. By dissecting the different steps of published eukaryotic and prokaryotic Hi-C protocols, we have developed a cost- and time-effective approach to generate high-resolution (down to 500 bp - 1 kb) contact matrices of both bacteria and archaea genomes.

Keywords: Genetics; Genomics; Microbiology; Molecular Biology; Sequencing

Domains: Microbiology and Parasitology

Charlotte Cockram, Agnès Thierry, Romain Koszul. Generation of gene-level resolution chromosome contact maps in bacteria and archaea. STAR Protocols, 2021, 2 (2), pp.100512. ⟨10.1016/j.xpro.2021.100512⟩. ⟨pasteur-03376845⟩