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Generation of gene-level resolution chromosome contact maps in bacteria and archaea

Abstract : Chromosome conformation capture (Hi-C) has become a routine method for probing the 3D organization of genomes. However, when applied to bacteria and archaea, current protocols are expensive and limited in their resolution. By dissecting the different steps of published eukaryotic and prokaryotic Hi-C protocols, we have developed a cost- and time-effective approach to generate high-resolution (down to 500 bp - 1 kb) contact matrices of both bacteria and archaea genomes.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03376845
Contributor : Romain Koszul Connect in order to contact the contributor
Submitted on : Wednesday, October 13, 2021 - 5:14:56 PM
Last modification on : Tuesday, November 23, 2021 - 2:38:07 PM

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Charlotte Cockram, Agnès Thierry, Romain Koszul. Generation of gene-level resolution chromosome contact maps in bacteria and archaea. STAR Protocols, Elsevier, 2021, 2 (2), pp.100512. ⟨10.1016/j.xpro.2021.100512⟩. ⟨pasteur-03376845⟩

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