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MetaHiC phage-bacteria infection network reveals active cycling phages of the healthy human gut

Abstract : Bacteriophages play important roles in regulating the intestinal human microbiota composition, dynamics, and homeostasis, and characterizing their bacterial hosts is needed to understand their impact. We applied a metagenomic Hi-C approach on 10 healthy human gut samples to unveil a large infection network encompassing more than 6000 interactions bridging a metagenomic assembled genomes (MAGs) and a phage sequence, allowing to study in situ phage-host ratio. Whereas three-quarters of these sequences likely correspond to dormant prophages, 5% exhibit a much higher coverage than their associated MAG, representing potentially actively replicating phages. We detected 17 sequences of members of the crAss-like phage family, whose hosts diversity remained until recently relatively elusive. For each of them, a unique bacterial host was identified, all belonging to different genus of Bacteroidetes. Therefore, metaHiC deciphers infection network of microbial population with a high specificity paving the way to dynamic analysis of mobile genetic elements in complex ecosystems.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03369735
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Submitted on : Thursday, October 7, 2021 - 3:14:26 PM
Last modification on : Saturday, October 9, 2021 - 4:03:18 AM

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Martial Marbouty, Agnès Thierry, Gael A. Millot, Romain Koszul. MetaHiC phage-bacteria infection network reveals active cycling phages of the healthy human gut. eLife, eLife Sciences Publication, 2021, 10, pp.e60608. ⟨10.7554/eLife.60608⟩. ⟨pasteur-03369735⟩

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