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Journal Articles Journal of Experimental Medicine Year : 2021

Implication of folate deficiency in CYP2U1 loss of function

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Serge Picaud
Alexandra Durr

Abstract

Hereditary spastic paraplegias are heterogeneous neurodegenerative disorders. Understanding of their pathogenic mechanisms remains sparse, and therapeutic options are lacking. We characterized a mouse model lacking the Cyp2u1 gene, loss of which is known to be involved in a complex form of these diseases in humans. We showed that this model partially recapitulated the clinical and biochemical phenotypes of patients. Using electron microscopy, lipidomic, and proteomic studies, we identified vitamin B2 as a substrate of the CYP2U1 enzyme, as well as coenzyme Q, neopterin, and IFN-α levels as putative biomarkers in mice and fluids obtained from the largest series of CYP2U1-mutated patients reported so far. We also confirmed brain calcifications as a potential biomarker in patients. Our results suggest that CYP2U1 deficiency disrupts mitochondrial function and impacts proper neurodevelopment, which could be prevented by folate supplementation in our mouse model, followed by a neurodegenerative process altering multiple neuronal and extraneuronal tissues.
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Origin : Publication funded by an institution

Dates and versions

pasteur-03349041 , version 1 (20-09-2021)

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Attribution - NonCommercial - ShareAlike - CC BY 4.0

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Claire Pujol, Anne Legrand, Livia Parodi, Priscilla Thomas, Fanny Mochel, et al.. Implication of folate deficiency in CYP2U1 loss of function. Journal of Experimental Medicine, 2021, 218 (11), pp.e20210846. ⟨10.1084/jem.20210846⟩. ⟨pasteur-03349041⟩
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