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Platelet activation in critically ill COVID-19 patients

Abstract : Background Microvascular, arterial and venous thrombotic events have been largely described during severe coronavirus disease 19 (COVID-19). However, mechanisms underlying hemostasis dysregulation remain unclear. Methods We explored two independent cross-sectional cohorts to identify soluble markers and gene-expression signatures that discriminated COVID-19 severity and outcomes. Results We found that elevated soluble (s)P-selectin at admission was associated with disease severity. Elevated sP-selectin was predictive of intubation and death (ROC AUC = 0.67, p = 0.028 and AUC = 0.74, p = 0.0047, respectively). An optimal cutoff value was predictive of intubation with 66% negative predictive value (NPV) and 61% positive predictive value (PPV), and of death with 90% NPV and 55% PPV. An unbiased gene set enrichment analysis revealed that critically ill patients had increased expression of genes related to platelet activation. Hierarchical clustering identified ITG2AB , GP1BB , PPBP and SELPLG to be upregulated in a grade-dependent manner. ROC curve analysis for the prediction of intubation was significant for SELPLG and PPBP (AUC = 0.8, p = 0.046 for both). An optimal cutoff value for PBPP was predictive of intubation with 100% NPV and 45% PPV, and for SELPLG with 100% NPV and 50% PPV. Conclusion We provide evidence that platelets contribute to COVID-19 severity. Plasma sP-selectin level was associated with severity and in-hospital mortality. Transcriptional analysis identified PPBP/CXCL7 and SELPLG as biomarkers for intubation. These findings provide additional evidence for platelet activation in driving critical COVID-19. Specific studies evaluating the performance of these biomarkers are required.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03319588
Contributor : Marie-Christine Vougny <>
Submitted on : Thursday, August 12, 2021 - 3:25:28 PM
Last modification on : Tuesday, August 17, 2021 - 6:05:49 PM

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Distributed under a Creative Commons Attribution 4.0 International License

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Nader Yatim, Jeremy Boussier, Richard Chocron, Jérôme Hadjadj, Aurélien Philippe, et al.. Platelet activation in critically ill COVID-19 patients. Annals of Intensive Care, SpringerOpen, 2021, 11 (1), pp.113. ⟨10.1186/s13613-021-00899-1⟩. ⟨pasteur-03319588⟩

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