Kinetics of the Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Response and Serological Estimation of Time Since Infection
(1, 2)
,
(1, 2)
,
(1, 2, 3)
,
(1, 2)
,
(1, 2)
,
(1, 2, 4)
,
(2)
,
(2)
,
(5, 6)
,
(6)
,
(7)
,
(7)
,
(7)
,
(8)
,
(9)
,
(10)
,
(11, 12)
,
(13, 14)
,
(15)
,
(16)
,
(16)
,
(17)
,
(17)
,
(18)
,
(18)
,
(19)
,
(19)
,
(20)
,
(21, 22)
,
(21, 22)
,
(21, 22)
,
(21, 22)
,
(23)
,
(23)
,
(24)
,
(24)
,
(10)
,
(25)
,
(24, 26)
,
(2, 27, 28)
,
(5)
,
(16, 29)
,
(1, 2)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
Stéphane Pelleau
- Function : Author
- PersonId : 751034
- IdHAL : stephane-pelleau
- ORCID : 0000-0002-7070-2177
Thomas Obadia
- Function : Author
- PersonId : 184834
- IdHAL : thomas-obadia
- IdRef : 156629321
Charlotte Cockram
- Function : Author
- PersonId : 800496
- ORCID : 0000-0003-2130-9210
Sarah Hélène Merkling
- Function : Author
- PersonId : 1074096
- IdHAL : sarahmerkling
- ORCID : 0000-0002-6823-4474
Annalisa Meola
- Function : Author
- PersonId : 754880
- IdHAL : annalisa-meola
- ORCID : 0000-0003-2582-4463
Benjamin Terrier
- Function : Author
- PersonId : 762205
- ORCID : 0000-0001-6612-7336
- IdRef : 13141142X
Delphine Planas
- Function : Author
- PersonId : 805693
- ORCID : 0000-0002-2509-9954
Olivier Schwartz
- Function : Author
- PersonId : 757442
- ORCID : 0000-0002-0729-1475
- IdRef : 128673850
Darragh Duffy
- Function : Author
- PersonId : 746816
- IdHAL : darragh-duffy
- ORCID : 0000-0002-8875-2308
- IdRef : 201316919
Veasna Duong
- Function : Author
- PersonId : 765625
- ORCID : 0000-0003-0353-1678
Bruno Hoen
- Function : Author
- PersonId : 11357
- IdHAL : bruno-hoen
- ORCID : 0000-0003-3070-3017
- IdRef : 058486194
Ivo Mueller
- Function : Author
- PersonId : 784421
- ORCID : 0000-0001-6554-6889
- IdRef : 241942551
Samira Fafi-Kremer
- Function : Author
- PersonId : 758654
- ORCID : 0000-0003-3886-7833
- IdRef : 094693501
Timothée Bruel
- Function : Author
- PersonId : 746191
- IdHAL : timothee-bruel
- ORCID : 0000-0002-3952-4261
- IdRef : 169340171
Michael White
Connectez-vous pour contacter l'auteur
- Function : Correspondent author
- PersonId : 1069413
Connectez-vous pour contacter l'auteur
Abstract
Background Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces a complex antibody response that varies by orders of magnitude between individuals and over time. Methods We developed a multiplex serological test for measuring antibodies to five SARS-CoV-2 antigens and the Spike proteins of seasonal coronaviruses. We measured antibody responses in cohorts of hospitalized patients and healthcare workers followed for up to eleven months after symptoms. A mathematical model of antibody kinetics was used to quantify the duration of antibody responses. Antibody response data were used to train algorithms for estimating time since infection. Results One year after symptoms, we estimate that 36% (95% range: 11%, 94%) of anti-Spike IgG remains, 31% (9%, 89%) anti-RBD IgG remains, and 7% (1%, 31%) anti-Nucleocapsid IgG remains. The multiplex assay classified previous infections into time intervals of 0–3 months, 3–6 months, and 6–12 months. This method was validated using data from a sero-prevalence survey in France, demonstrating that historical SARS-CoV-2 transmission can be reconstructed using samples from a single survey. Conclusions In addition to diagnosing previous SARS-CoV-2 infection, multiplex serological assays can estimate the time since infection which can be used to reconstruct past epidemics.
Origin : Publication funded by an institution