Skip to Main content Skip to Navigation
Journal articles

Structural and catalytic properties and homology modelling of the human nucleoside diphosphate kinase C, product of the DRnm23 gene

Abstract : The human DRnm23 gene was identified by differential screening of a cDNA library obtained from chronic myeloid leukaemia-blast crisis primary cells. The over-expression of this gene inhibits differentiation and induces the apoptosis of myeloid precursor cell lines. We overproduced in bacteria a truncated form of the encoded protein lacking the first 17 N-terminal amino acids. This truncated protein was called nucleoside diphosphate (NDP) kinase CDelta. NDP kinase CDelta had similar kinetic properties to the major human NDP kinases A and B, but was significantly more stable to denaturation by urea and heat. Analysis of denaturation by urea, using size exclusion chromatography, indicated unfolding without the dissociation of subunits, whereas renaturation occurred via a folded monomer. The stability of the protein depended primarily on subunit interactions. Homology modelling of the structure of NDP kinase CDelta, based on the crystal structure of NDP kinase B, indicated that NDP kinase CDelta had several additional stabilizing interactions. The overall structure of the two enzymes appears to be identical because NDP kinase CDelta readily formed mixed hexamers with NDP kinase A. It is possible that mixed hexamers can be observed in vivo.
Document type :
Journal articles
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03276972
Contributor : Hélène Ribierre Connect in order to contact the contributor
Submitted on : Friday, July 2, 2021 - 3:31:53 PM
Last modification on : Tuesday, July 13, 2021 - 3:26:50 AM

Links full text

Identifiers

Citation

Muriel Erent, Philippe Gonin, Jacqueline Cherfils, Pierre Tissier, Giuseppe Raschellà, et al.. Structural and catalytic properties and homology modelling of the human nucleoside diphosphate kinase C, product of the DRnm23 gene. European Journal of Biochemistry, 2001, 268 (7), pp.1972-1981. ⟨10.1046/j.1432-1327.2001.2076.doc.x⟩. ⟨pasteur-03276972⟩

Share

Metrics

Record views

30