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The mechanism of phosphorylation of anti-HIV D4T by nucleoside diphosphate kinase

Abstract : The last step in the intracellular activation of antiviral nucleoside analogs is the addition of the third phosphate by nucleoside diphosphate (NDP) kinase resulting in the synthesis of the viral reverse transcriptase substrates. We have previously shown that dideoxynucleotide analogs and 3'-deoxy-3'-azidothymidine (AZT) as di- or triphosphate are poor substrates for NDP kinase. By use of protein fluorescence, we monitor the phosphotransfer between the enzyme and the nucleotide analog. Here, we have studied the reactivity of D4T (2',3'-dideoxy-2',3'-didehydrothymidine; stavudine) as di- (DP) or triphosphate (TP) at the pre-steady state. The catalytic efficiency of D4T-DP or -TP is increased by a factor of 10 compared with AZT-DP or -TP, respectively. We use an inactive mutant of NDP kinase to monitor the binding of a TP derivative, and show that the affinity for D4T-TP is in the same range as for the natural substrate deoxythymidine triphosphate, but is 30 times higher than for AZT-TP. Our results indicate that D4T should be efficiently phosphorylated after intracellular maturation of a prodrug into D4T-monophosphate.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03276940
Contributor : Hélène Ribierre <>
Submitted on : Friday, July 2, 2021 - 3:19:05 PM
Last modification on : Sunday, July 4, 2021 - 11:50:03 AM

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  • HAL Id : pasteur-03276940, version 1
  • PUBMED : 10779378

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Benoit Schneider, Ricardo Biondi, Robert Sarfati, Fabrice Agou, Catherine Guerreiro, et al.. The mechanism of phosphorylation of anti-HIV D4T by nucleoside diphosphate kinase. Molecular Pharmacology, American Society for Pharmacology and Experimental Therapeutics, 2000, 57 (5), pp.948-53. ⟨pasteur-03276940⟩

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