Skip to Main content Skip to Navigation
Journal articles

The in vitro DNA binding properties of NDP kinase are related to its oligomeric state

Abstract : Genetic and biochemical evidences suggest that the enzymatic activity of NDP kinase is necessary but not sufficient for its biological function. While the human NDPK-B binds specifically single-strand polypyrimidines sequences, the hexameric enzyme from Dictyostelium does not. We demonstrated by electrophoretic mobility shift assay and filter binding assay that a dimeric mutant from Dictyostelium binds to an oligodesoxynucleotide while the wild-type does not. These data suggest that the differences in the DNA binding properties of several eucaryotic NDP kinases might be correlated to the differences in the stability of their hexameric structure.
Document type :
Journal articles
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03276726
Contributor : Hélène Ribierre Connect in order to contact the contributor
Submitted on : Friday, July 2, 2021 - 12:49:08 PM
Last modification on : Tuesday, October 19, 2021 - 10:27:17 PM

Links full text

Identifiers

Collections

Citation

Sébastien Mesnildrey, Fabrice Agou, Michel Véron. The in vitro DNA binding properties of NDP kinase are related to its oligomeric state. FEBS Letters, Wiley, 1997, 418 (1-2), pp.53-57. ⟨10.1016/s0014-5793(97)01292-1⟩. ⟨pasteur-03276726⟩

Share

Metrics