T cells regulate lymph node-resident ILC populations in a tissue and subset-specific way

Priscillia Bresler (1) , Emmanuel Tejerina (1) , Jean Marie Jacob (2, 3) , Agnès Legrand (1) , Véronique Quellec (1) , Sophie Ezine (1) , Lucie Peduto (2) , Marie Cherrier (4)

1 INEM - UM 111 (UMR 8253 / U1151) - Institut Necker Enfants-Malades
2 Stroma, inflammation et réparation tissulaire - Stroma, Inflammation and Tissue Repair
3 Université Paris Diderot, Sorbonne Paris Cité, Paris, France
4 Equipe Inserm U1163 - Laboratory of Intestinal Immunity

Innate lymphoid cells (ILCs) have been shown to be significantly affected in the small intestine lamina propria and secondary lymphoid organs (SLOs) of conventional lymphopenic mice. How ILCs are regulated by adaptive immunity in SLOs remains unclear. In T cell-deficient mice, ILC2s are significantly increased in the mesenteric lymph nodes (MLNs) at the expense of CCR6+ ILC3s, which are nonetheless increased in the peripheral lymph nodes (PLNs). Here, we show that T cells regulate lymph node-resident ILCs in a tissue- and subset-specific way. First, reducing microbial colonization from birth restored CCR6+ ILC3s in the MLNs of T cell-deficient mice. In contrast, T cell reconstitution resulted in the contraction of both MLNs ILC2s and PLNs ILC3s, whereas antagonizing microbial colonization from birth had no impact on these populations. Finally, the accumulation of MLNs ILC2s was partly regulated by T cells through stroma-derived IL-33.

Mots clés: Microbiome; Components of the Immune System; Immunology

Domaines: Sciences du Vivant [q-bio]

Priscillia Bresler, Emmanuel Tejerina, Jean Marie Jacob, Agnès Legrand, Véronique Quellec, et al.. T cells regulate lymph node-resident ILC populations in a tissue and subset-specific way. iScience, 2021, 24 (3), pp.102158. ⟨10.1016/j.isci.2021.102158⟩. ⟨pasteur-03264565⟩