Evolution and function of bacterial RCC1 repeat effectors - Archive ouverte HAL Access content directly
Journal Articles Cellular Microbiology Year : 2020

Evolution and function of bacterial RCC1 repeat effectors

(1) , (2) , (2) , (1)
1
2

Abstract

Intracellular bacterial pathogens harbour genes, the closest homologues of which are found in eukaryotes. Regulator of chromosome condensation 1 (RCC1) repeat proteins are phylogenetically widespread and implicated in protein-protein interactions, such as the activation of the small GTPase Ran by its cognate guanine nucleotide exchange factor, RCC1. Legionella pneumophila and Coxiella burnetii, the causative agents of Legionnaires' disease and Q fever, respectively, harbour RCC1 repeat coding genes. Legionella pneumophila secretes the RCC1 repeat 'effector' proteins LegG1, PpgA and PieG into eukaryotic host cells, where they promote the activation of the pleiotropic small GTPase Ran, microtubule stabilisation, pathogen vacuole motility and intracellular bacterial growth as well as host cell migration. The RCC1 repeat effectors localise to the pathogen vacuole or the host plasma membrane and target distinct components of the Ran GTPase cycle, including Ran modulators and the small GTPase itself. Coxiella burnetii translocates the RCC1 repeat effector NopA into host cells, where the protein localises to nucleoli. NopA binds to Ran GTPase and promotes the nuclear accumulation of Ran(GTP), thus pertubing the import of the transcription factor NF-κB and innate immune signalling. Hence, divergent evolution of bacterial RCC1 repeat effectors defines the range of Ran GTPase cycle targets and likely allows fine-tuning of Ran GTPase activation by the pathogens at different cellular sites.
Fichier principal
Vignette du fichier
2020 Cell Microbiol_Swart.pdf (701.7 Ko) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

pasteur-03264023 , version 1 (17-06-2021)

Licence

Attribution - NonCommercial - CC BY 4.0

Identifiers

Cite

Anna Leoni Swart, Laura Gomez-Valero, Carmen Buchrieser, Hubert Hilbi. Evolution and function of bacterial RCC1 repeat effectors. Cellular Microbiology, 2020, 22 (10), pp.e13246. ⟨10.1111/cmi.13246⟩. ⟨pasteur-03264023⟩
58 View
32 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More