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Salmonella enters a dormant state within human epithelial cells for persistent infection

Abstract : Salmonella Typhimurium ( S . Typhimurium) is an enteric bacterium capable of invading a wide range of hosts, including rodents and humans. It targets different host cell types showing different intracellular lifestyles. S . Typhimurium colonizes different intracellular niches and is able to either actively divide at various rates or remain dormant to persist. A comprehensive tool to determine these distinct S . Typhimurium lifestyles remains lacking. Here we developed a novel fluorescent reporter, Salmonella INtracellular Analyzer (SINA), compatible for fluorescence microscopy and flow cytometry in single-bacterium level quantification. This identified a S . Typhimurium subpopulation in infected epithelial cells that exhibits a unique phenotype in comparison to the previously documented vacuolar or cytosolic S . Typhimurium. This subpopulation entered a dormant state in a vesicular compartment distinct from the conventional Salmonella -containing vacuoles (SCV) as well as the previously reported niche of dormant S . Typhimurium in macrophages. The dormant S . Typhimurium inside enterocytes were viable and expressed Salmonella Pathogenicity Island 2 (SPI-2) virulence factors at later time points. We found that the formation of these dormant S . Typhimurium is not triggered by the loss of SPI-2 effector secretion but it is regulated by (p)ppGpp-mediated stringent response through RelA and SpoT. We predict that intraepithelial dormant S . Typhimurium represents an important pathogen niche and provides an alternative strategy for S . Typhimurium pathogenicity and its persistence.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03263221
Contributor : Laurence Langlais Connect in order to contact the contributor
Submitted on : Thursday, June 17, 2021 - 10:03:06 AM
Last modification on : Thursday, April 7, 2022 - 1:58:13 PM
Long-term archiving on: : Saturday, September 18, 2021 - 6:11:40 PM

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Chak Hon Luk, Camila Valenzuela, Magdalena Gil, Léa Swistak, Perrine Bomme, et al.. Salmonella enters a dormant state within human epithelial cells for persistent infection. PLoS Pathogens, Public Library of Science, 2021, 17 (4), pp.e1009550. ⟨10.1371/journal.ppat.1009550⟩. ⟨pasteur-03263221⟩

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