HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Heterogeneous expression of Pil3 pilus is critical for Streptococcus gallolyticus translocation across polarized colonic epithelial monolayers

Abstract : Streptococcus gallolyticus is an opportunistic pathogen responsible for septicemia and endocarditis. We report that S. gallolyticus UCN34 adheres and crosses epithelial monolayers in a Pil3 dependent manner. Confocal images revealed a paracellular passage. Both the Δpil3 mutant and the Pil3+ overexpressing variant were unable to cross Caco-2 and T84 barriers. However, combining live Δpil3 mutant with fixed Pil3+ variant in a 9:1 ratio allowed efficient translocation of the Δpil3 mutant. These results demonstrate that heterogeneous expression of Pil3 plays a key role for UCN34 translocation across the intestinal barrier. Through this skilful strategy, S. gallolyticus probably evade host immune responses.
Document type :
Journal articles
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03262258
Contributor : Shaynoor Dramsi Connect in order to contact the contributor
Submitted on : Wednesday, June 16, 2021 - 1:51:15 PM
Last modification on : Wednesday, May 11, 2022 - 5:08:06 PM
Long-term archiving on: : Friday, September 17, 2021 - 6:51:06 PM

Licence


Distributed under a Creative Commons Attribution - NoDerivatives 4.0 International License

Identifiers

Collections

Citation

Mariana Martins, Laurence Du Merle, Patrick Trieu-Cuot, Shaynoor Dramsi. Heterogeneous expression of Pil3 pilus is critical for Streptococcus gallolyticus translocation across polarized colonic epithelial monolayers. Microbes and Infection, Elsevier, 2020, 22 (1), pp.55-59. ⟨10.1016/j.micinf.2019.12.001⟩. ⟨pasteur-03262258⟩

Share

Metrics

Record views

8

Files downloads

32