Antigen-Specific In Vivo Killing Assay

Nada Chaoul (1) , Gilles Dadaglio (2)

1 UNIBA - Università degli studi di Bari Aldo Moro = University of Bari Aldo Moro
2 Immunobiologie de l'Infection - Immunobiology of Infection

The in vivo killing assay allows the quantification of the antigen-specific killing capacity of Cytotoxic CD8+ T Lymphocytes (CTLs) in mice. CTLs are indeed known for the lysis of cells expressing foreign or modified antigen peptides on their MHC class I molecules. Here we describe the detailed protocol used for the in vivo specific lysis of cells expressing the H-2 Kb immunodominant CD8+ T-cell epitope of the OVA protein: an 8 amino acid peptide corresponding to the 257-264 region of OVA (SIINFEKL).

Mots clés: Antigen-specific lysis of target cells; Cytotoxic CD8+ T lymphocytes; Flow cytometry-based In vivo killing assay; Mouse model; Vaccine testing

Domaines: Immunité adaptative

Nada Chaoul, Gilles Dadaglio. Antigen-Specific In Vivo Killing Assay. Margherita Gigante; Elena Ranieri. Cytotoxic T-Cells: methods and protocols, 2325, Sringer, pp.55-64, 2021, Methods in Molecular Biology, 978-1-0716-1507-2 (e-book). ⟨10.1007/978-1-0716-1507-2_4⟩. ⟨pasteur-03261883⟩