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Antigen-Specific In Vivo Killing Assay

Abstract : The in vivo killing assay allows the quantification of the antigen-specific killing capacity of Cytotoxic CD8+ T Lymphocytes (CTLs) in mice. CTLs are indeed known for the lysis of cells expressing foreign or modified antigen peptides on their MHC class I molecules. Here we describe the detailed protocol used for the in vivo specific lysis of cells expressing the H-2 Kb immunodominant CD8+ T-cell epitope of the OVA protein: an 8 amino acid peptide corresponding to the 257-264 region of OVA (SIINFEKL).
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Contributor : Gilles Dadaglio Connect in order to contact the contributor
Submitted on : Wednesday, June 16, 2021 - 10:47:46 AM
Last modification on : Tuesday, April 19, 2022 - 11:48:01 AM




Nada Chaoul, Gilles Dadaglio. Antigen-Specific In Vivo Killing Assay. Margherita Gigante; Elena Ranieri. Cytotoxic T-Cells: methods and protocols, 2325, Sringer, pp.55-64, 2021, Methods in Molecular Biology, 978-1-0716-1507-2 (e-book). ⟨10.1007/978-1-0716-1507-2_4⟩. ⟨pasteur-03261883⟩



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