Diagnosis of Meningococcal Infection Using Internally Controlled Multiplex Real-Time PCR - Archive ouverte HAL Access content directly
Book Sections Year : 2019

Diagnosis of Meningococcal Infection Using Internally Controlled Multiplex Real-Time PCR

(1) , (1) , (1)
1

Abstract

Neisseria meningitidis (Nm) is a leading cause of invasive infections associated with high mortality and morbidity, notably meningitis and septicemia. Etiological rapid diagnosis is key for the preventive management of invasive meningococcal disease (IMD). However, conventional methods for diagnosis are timeconsuming and could be hampered by the difficulties in culturing the isolates from clinical specimens especially due to early antibiotic treatment. Therefore, sensitive, specific and rapid non-culture-based methods are valuable for early diagnosis, effective therapy, and prevention. Here we describe a real-time PCR multiplex assays for the detection of Nm targeting the meningococcal-specific gene crgA, coding for a LysR-like transcriptional regulator, and six serogroup-specific (A, B, C, W, X, Y) Nm capsular genes, using a Qiagen column-based method for the optimum isolation of DNA from clinical specimens. Internal quality controls were included to monitor extraction of DNA, inhibition and the technical validation of the PCR as well.
Not file

Dates and versions

pasteur-03261589 , version 1 (15-06-2021)

Identifiers

Cite

Ala-Eddine Deghmane, Eva Hong, Muhamed-Kheir Taha. Diagnosis of Meningococcal Infection Using Internally Controlled Multiplex Real-Time PCR. Neisseria meningitidis, 1969, Humana Press, New York, NY, pp.17-31, 2019, Methods in Molecular Biology, 978-1-4939-9201-0. ⟨10.1007/978-1-4939-9202-7_2⟩. ⟨pasteur-03261589⟩

Collections

PASTEUR
7 View
3 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More