Developing a tetO/TetR system in Neurospora crassa - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Fungal Genetics and Biology Année : 2020

Developing a tetO/TetR system in Neurospora crassa

Résumé

The development of a tetO/TetR system in the fungus Neurospora crassa is described. The system includes (i) a synthetic gene encoding a TetR variant fused to GFP, and (ii) a standard tetO array integrated homologously, as a proof of principle, near the his-3 gene. The localization of TetR-GFP at the tetO array (observed by fluorescence microscopy) can be disrupted by the application of tetracycline. The full-length array is stable during vegetative growth, but it triggers strong repeat-induced point mutation (RIP) by the RID-dependent as well as the DIM-2-dependent pathways during the sexual phase. Thus, both RIP pathways must be inactivated to allow the faithful inheritance of the unmodified construct. In summary, this study introduces a new molecular tool into Neurospora research, and suggests that the standard tetO array can self-engage in recombination-independent homologous pairing.
Fichier principal
Vignette du fichier
S1087184519303615.pdf (1.3 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

pasteur-03261456 , version 1 (21-07-2022)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Tinh-Suong Nguyen, Eugene Gladyshev. Developing a tetO/TetR system in Neurospora crassa. Fungal Genetics and Biology, 2020, 136, pp.103316. ⟨10.1016/j.fgb.2019.103316⟩. ⟨pasteur-03261456⟩

Collections

PASTEUR ANR FRM
22 Consultations
59 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More