Skip to Main content Skip to Navigation
Journal articles

Zika Virus Subgenomic Flavivirus RNA Generation Requires Cooperativity between Duplicated RNA Structures That Are Essential for Productive Infection in Human Cells

Abstract : Flaviviruses are important emerging and reemerging human pathogens. Understanding the molecular mechanisms for viral replication and evasion of host antiviral responses is relevant to development of control strategies. Flavivirus infections produce viral noncoding RNAs, known as sfRNAs, involved in viral replication and pathogenesis. In this study, we dissected molecular determinants for Zika virus sfRNA generation in the two natural hosts, human cells and mosquitoes. We found that two RNA structures of the viral 3′ UTR operate in a cooperative manner to produce two species of sfRNAs and that the deletion of these elements has a profoundly different impact on viral replication in the two hosts. Generation of at least one sfRNA was necessary for efficient Zika virus infection of Aedes aegypti mosquitoes. Moreover, recombinant viruses with different 3′ UTR arrangements revealed an essential role of sfRNAs for productive infection in human cells. In summary, we define molecular requirements for Zika virus sfRNA accumulation and provide new ideas of how flavivirus RNA structures have evolved to succeed in different hosts.
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03260681
Contributor : Brigitte Bidault Connect in order to contact the contributor
Submitted on : Tuesday, June 15, 2021 - 10:18:07 AM
Last modification on : Tuesday, October 19, 2021 - 10:28:45 PM

Links full text

Identifiers

Collections

Citation

Horacio Pallarés, Guadalupe Soledad Costa Navarro, Sergio Villordo, Fernando Merwaiss, Luana de Borba, et al.. Zika Virus Subgenomic Flavivirus RNA Generation Requires Cooperativity between Duplicated RNA Structures That Are Essential for Productive Infection in Human Cells. Journal of Virology, American Society for Microbiology, 2020, 94 (18), ⟨10.1128/JVI.00343-20⟩. ⟨pasteur-03260681⟩

Share

Metrics