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Pseudomonas carbonaria sp. nov., isolated from charcoal

Abstract : A beige-pigmented, oxidase-positive bacterial isolate, Wesi-4 T , isolated from charcoal in 2012, was examined in detail by applying a polyphasic taxonomic approach. Cells of the isolates were rod shaped and Gram-stain negative. Examination of the 16S rRNA gene sequence of the isolate revealed highest sequence similarities to the type strains of Pseudomonas matsuisoli and Pseudomonas nosocomialis (both 97.3 %). Phylogenetic analyses on the basis of the 16S rRNA gene sequences indicated a separate position of Wesi-4 T , which was confirmed by multilocus sequence analyses (MLSA) based on the three loci gyrB , rpoB and rpoD and a core genome-based phylogenetic tree. Genome sequence based comparison of Wesi-4 T and the type strains of P. matsuisoli and P. nosocomialis yielded average nucleotide identity values <95 % and in silico DNA-DNA hybridization values <70 %, respectively. The polyamine pattern contains the major amines putrescine, cadaverine and spermidine. The quinone system contains predominantly ubiquinone Q-9 and in the polar lipid profile diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine are the major lipids. The fatty acid contains predominantly C 16 : 0 , summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ) and summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ). In addition, physiological and biochemical tests revealed a clear phenotypic difference from P. matsuisoli . These cumulative data indicate that the isolate represents a novel species of the genus Pseudomonas for which the name Pseudomonas carbonaria sp. nov. is proposed with Wesi-4 T (=DSM 110367 T =CIP 111764 T =CCM 9017 T ) as the type strain.
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Submitted on : Monday, June 14, 2021 - 2:05:40 PM
Last modification on : Tuesday, October 19, 2021 - 10:28:45 PM

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Peter Kämpfer, S. Glaeser, John Mcinroy, Dominique Clermont, Alexis Criscuolo, et al.. Pseudomonas carbonaria sp. nov., isolated from charcoal. International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2021, 71 (4), ⟨10.1099/ijsem.0.004750⟩. ⟨pasteur-03259701⟩

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