HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

ERM-Dependent Assembly of T Cell Receptor Signaling and Co-stimulatory Molecules on Microvilli prior to Activation

Abstract : T cell surfaces are covered with microvilli, actin-rich and flexible protrusions. We use super-resolution microscopy to show that ≥90% of T cell receptor (TCR) complex molecules TCRαβ and TCRζ, as well as the co-receptor CD4 (cluster of differentiation 4) and the co-stimulatory molecule CD2, reside on microvilli of resting human T cells. Furthermore, TCR proximal signaling molecules involved in the initial stages of the immune response, including the protein tyrosine kinase Lck (lymphocyte-specific protein tyrosine kinase) and the key adaptor LAT (linker for activation of T cells), are also enriched on microvilli. Notably, phosphorylated proteins of the ERM (ezrin, radixin, and moesin) family colocalize with TCRαβ as well as with actin filaments, implying a role for one or more ERMs in linking the TCR complex to the actin cytoskeleton within microvilli. Our results establish microvilli as key signaling hubs, in which the TCR complex and its proximal signaling molecules and adaptors are preassembled prior to activation in an ERM-dependent manner, facilitating initial antigen sensing.
Document type :
Journal articles
Complete list of metadata

Contributor : Vincenzo Di Bartolo Connect in order to contact the contributor
Submitted on : Friday, June 11, 2021 - 4:30:13 PM
Last modification on : Thursday, April 7, 2022 - 10:10:45 AM
Long-term archiving on: : Sunday, September 12, 2021 - 8:11:00 PM


Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License




Shirsendu Ghosh, Vincenzo Di Bartolo, Liron Tubul, Eyal Shimoni, Elena Kartvelishvily, et al.. ERM-Dependent Assembly of T Cell Receptor Signaling and Co-stimulatory Molecules on Microvilli prior to Activation. Cell Reports, Elsevier Inc, 2020, 30 (10), pp.3434-3447.e6. ⟨10.1016/j.celrep.2020.02.069⟩. ⟨pasteur-03258643⟩



Record views


Files downloads