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Journal articles
A single-shot Lassa vaccine induces long-term immunity and protects cynomolgus monkeys against heterologous strains
Mathieu Mateo
1
Stéphanie Reynard
1
Alexandra Journeaux
1
Clara Germain
1
Jimmy Hortion
1
Xavier Carnec
1
Caroline Picard
1
Nicolas Baillet
1
Virginie Borges-Cardoso
1
Othmann Merabet
1
Audrey Vallve
2
Stéphane Barron
2
Ophélie Jourjon
2
Orianne Lacroix
2
Aurélie Duthey
2
Manon Dirheimer
3
Gregory Jouvion
4, 5, 6
Pierre-Henri Moreau
7
Lyne Fellmann
7
Caroline Carbonnelle
2
Hervé Raoul
2
Frédéric Tangy
8
Sylvain Baize
1, *
*
Corresponding author
Mathieu Mateo 1
Author
Stéphanie Reynard 1
Author
Alexandra Journeaux 1
Author
Clara Germain 1
Author
Virginie Borges-Cardoso 1
Author
Othmann Merabet 1
Author
Audrey Vallve 2
Author
Stéphane Barron 2
Author
Gregory Jouvion 4, 5, 6
Author
Pierre-Henri Moreau 7
Author
Lyne Fellmann 7
Author
Caroline Carbonnelle 2
Author
Hervé Raoul 2
Author
Frédéric Tangy 8
Author
Sylvain Baize 1
Correspondent author IdHAL : sylvain-baize
1
UBIVE - Biologie des Infections Virales Émergentes - Biology of Emerging Viral Infections (21 avenue Tony Garnier 69365 Lyon Cedex 07 - France)
- CIRI - Centre International de Recherche en Infectiologie - UMR (21 avenue Tony Garnier 69365 Lyon Cedex 07 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1111 (101, rue de Tolbiac, 75013 Paris - France)
- ENS Lyon - École normale supérieure - Lyon (15 parvis René Descartes - BP 7000 - 69342 Lyon Cedex 07 - France)
- UCBL - Université Claude Bernard Lyon 1 (43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex - France)
- Université de Lyon (92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5308 (France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
2
Laboratoire P4 - Jean Mérieux (21 avenue Tony Garnier 69365 Lyon Cedex 07 - France)
- Centre Européen de Virologie/Immunologie (France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : US3 (101, rue de Tolbiac, 75013 Paris - France)
3
INSERM - Institut National de la Santé et de la Recherche Médicale (101, rue de Tolbiac, 75013 Paris - France)
4
ENVA - École nationale vétérinaire d'Alfort (7 avenue du Général de Gaulle - 94700 Maisons Alfort - France)
5
UPEC UP12 - Université Paris-Est Créteil Val-de-Marne - Paris 12 (61 avenue du Général de Gaulle - 94010 Créteil cedex - France)
6
ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (14 rue Pierre et Marie Curie
94701 MAISONS-ALFORT Cedex - France)
7
UNISTRA - Université de Strasbourg (4 rue Blaise Pascal - CS 90032 - 67081 Strasbourg cedex - France)
8
Génomique Virale et Vaccination (25-28 rue du Docteur Roux, F-75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : URA3015 (France)
Abstract : A safe and protective Lassa virus vaccine is crucially needed in Western Africa to stem the recurrent outbreaks of Lassa virus infections in Nigeria and the emergence of Lassa virus in previously unaffected countries, such as Benin and Togo. Major challenges in developing a Lassa virus vaccine include the high diversity of circulating strains and their reemergence from 1 year to another. To address each of these challenges, we immunized cynomolgus monkeys with a measles virus vector expressing the Lassa virus glycoprotein and nucleoprotein of the prototypic Lassa virus strain Josiah (MeV-NP). To evaluate vaccine efficacy against heterologous strains of Lassa virus, we challenged the monkeys a month later with heterologous strains from lineage II or lineage VII, finding that the vaccine was protective against these strains. A second cohort of monkeys was challenged 1 year later with the homologous Josiah strain, finding that a single dose of MeV-NP was sufficient to protect all vaccinated monkeys. These studies demonstrate that MeV-NP can generate both long-lasting immune responses and responses that are able to protect against diverse strains of Lassa virus.
Document type :
Journal articles
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Complete list of metadata
https://hal-pasteur.archives-ouvertes.fr/pasteur-03256146
Contributor : Sylvain Baize Connect in order to contact the contributor
Submitted on : Thursday, June 10, 2021 - 9:56:33 AM
Last modification on : Tuesday, October 19, 2021 - 10:27:03 PM
Long-term archiving on: : Saturday, September 11, 2021 - 6:17:35 PM
Contributor : Sylvain Baize Connect in order to contact the contributor
Submitted on : Thursday, June 10, 2021 - 9:56:33 AM
Last modification on : Tuesday, October 19, 2021 - 10:27:03 PM
Long-term archiving on: : Saturday, September 11, 2021 - 6:17:35 PM
Licence
Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License
Identifiers
- HAL Id : pasteur-03256146, version 1
- DOI : 10.1126/scitranslmed.abf6348
- PUBMED : 34108251
Collections
PASTEUR | ANSES | UPEC-UPEM | UPEC | ENS-LYON | UNIV-LYON1 | CIRI | CNRS | UDL | SITE-ALSACE | CIRI-S-BAIZE-UBIVE | INSERM
Citation
Mathieu Mateo, Stéphanie Reynard, Alexandra Journeaux, Clara Germain, Jimmy Hortion, et al.. A single-shot Lassa vaccine induces long-term immunity and protects cynomolgus monkeys against heterologous strains. Science Translational Medicine, American Association for the Advancement of Science, 2021, 13 (597), pp.eabf6348. ⟨10.1126/scitranslmed.abf6348⟩. ⟨pasteur-03256146⟩
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