BMP4 patterns Smad activity and generates stereotyped cell fate organisation in spinal organoids - Archive ouverte HAL Access content directly
Journal Articles Development (Cambridge, England) Year : 2019

BMP4 patterns Smad activity and generates stereotyped cell fate organisation in spinal organoids

(1, 2) , (3, 4) , (1) , (1) , (1, 5) , (1, 5) , (3, 4) , (1)
1
2
3
4
5

Abstract

Bone Morphogenetic Proteins (BMP) are secreted regulators of cell fate in several developing tissues. In the embryonic spinal cord, they control the emergence of the neural crest, roof plate and distinct subsets of dorsal interneurons. Although a gradient of BMP activity has been proposed to determine cell type identity in vivo, whether this is sufficient for pattern formation in vitro is unclear. Here, we demonstrate that exposure to BMP4 initiates distinct spatial dynamics of BMP signalling within the self-emerging epithelia of both mouse and human pluripotent stem cells derived spinal organoids. The pattern of BMP signalling results in the stereotyped spatial arrangement of dorsal neural tube cell types and concentration, timing and duration of BMP4 exposure modulate these patterns. Moreover, differences in the duration of competence time-windows between mouse and human account for the species specific tempo of neural differentiation. Together the study describes efficient methods to generate patterned subsets of dorsal interneurons in spinal organoids and supports the conclusion that graded BMP activity orchestrates the spatial organization of the dorsal neural tube cellular diversity in mouse and human.
Fichier principal
Vignette du fichier
dev175430.pdf (8.78 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

pasteur-03254035 , version 1 (08-06-2021)

Licence

Copyright

Identifiers

Cite

Nathalie Duval, Célia Vaslin, Tiago Barata, Youcef Frarma, Vincent Contremoulins, et al.. BMP4 patterns Smad activity and generates stereotyped cell fate organisation in spinal organoids. Development (Cambridge, England), 2019, 146 (14), pp.dev175430. ⟨10.1242/dev.175430⟩. ⟨pasteur-03254035⟩
24 View
28 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More