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Characterization of Endogenous SERINC5 Protein as Anti-HIV-1 Factor

Abstract : SERINC5 is the long-searched-for antiviral factor that is counteracted by the HIV-1 accessory gene product Nef. Here, we engineered, via CRISPR/Cas9 technology, T-cell lines that express endogenous SERINC5 alleles tagged with a knocked-in HA epitope. This genetic modification enabled us to study basic properties of endogenous SERINC5 and to verify proposed mechanisms of HIV-1 Nef-mediated counteraction of SERINC5. Using this unique resource, we identified the susceptibility of endogenous SERINC5 protein to posttranslational modulation by type I IFNs and suggest uncoupling of Nef-mediated functional antagonism from SERINC5 exclusion from virions.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03253574
Contributor : Nicoletta Casartelli <>
Submitted on : Tuesday, June 8, 2021 - 1:01:21 PM
Last modification on : Monday, June 14, 2021 - 5:14:02 PM

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Vânia Passos, Thomas Zillinger, Nicoletta Casartelli, Amelie Wachs, Shuting Xu, et al.. Characterization of Endogenous SERINC5 Protein as Anti-HIV-1 Factor. Journal of Virology, American Society for Microbiology, 2019, 93 (24), pp.e01221-19. ⟨10.1128/JVI.01221-19⟩. ⟨pasteur-03253574⟩

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