Characterization of Endogenous SERINC5 Protein as Anti-HIV-1 Factor

Vânia Passos; Thomas Zillinger; Nicoletta Casartelli; Amelie Wachs; Shuting Xu; Angelina Malassa; Katja Steppich; Hildegard Schilling; Sergej Franz; Daniel Todt; Eike Steinmann; Kathrin Sutter; Ulf Dittmer; Jens Bohne; Olivier Schwartz; Winfried Barchet; Christine Goffinet

doi:10.1128/JVI.01221-19

Journal articles

Characterization of Endogenous SERINC5 Protein as Anti-HIV-1 Factor

Vânia Passos ^{1, 2} Thomas Zillinger ³ Nicoletta Casartelli ⁴ Amelie Wachs ⁵ Shuting Xu ¹ Angelina Malassa ¹ Katja Steppich ¹ Hildegard Schilling ³ Sergej Franz ¹ Daniel Todt ⁶ Eike Steinmann ⁶ Kathrin Sutter ⁷ Ulf Dittmer ⁷ Jens Bohne ⁵ Olivier Schwartz ⁴ Winfried Barchet ^{3, 8} Christine Goffinet ^{1, 9, 10, *}

* Corresponding author

1 TWINCORE - Centre for Experimental and Clinical Infection Research [Hanover]

2 ICBAS - Instituto de Ciências Biomédicas de Abel Salazar

3 Institute of Clinical Chemistry and Clinical Pharmacology [Bonn, Allemagne]

4 Virus et Immunité - Virus and immunity

5 MHH - Hannover Medical School [Hannover]

6 RUB - Ruhr University Bochum

7 University of Duisburg-Essen

8 DZIF - German Centre for Infection Research

9 Charité - Universitätsmedizin Berlin / Charite - University Medicine Berlin

10 BIH - Berlin Institute of Health

Abstract : SERINC5 is the long-searched-for antiviral factor that is counteracted by the HIV-1 accessory gene product Nef. Here, we engineered, via CRISPR/Cas9 technology, T-cell lines that express endogenous SERINC5 alleles tagged with a knocked-in HA epitope. This genetic modification enabled us to study basic properties of endogenous SERINC5 and to verify proposed mechanisms of HIV-1 Nef-mediated counteraction of SERINC5. Using this unique resource, we identified the susceptibility of endogenous SERINC5 protein to posttranslational modulation by type I IFNs and suggest uncoupling of Nef-mediated functional antagonism from SERINC5 exclusion from virions.

Keywords : CRISPR/Cas9 HIV-1 Nef SERINC5 antiviral factor human immunodeficiency virus interferons

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Microbiology and Parasitology / Virology

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https://hal-pasteur.archives-ouvertes.fr/pasteur-03253574
Contributor : Nicoletta Casartelli <>
Submitted on : Tuesday, June 8, 2021 - 1:01:21 PM
Last modification on : Monday, June 14, 2021 - 5:14:02 PM
Long-term archiving on: : Thursday, September 9, 2021 - 7:06:46 PM

Characterization of Endogenous SERINC5 Protein as Anti-HIV-1 Factor

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Characterization of Endogenous SERINC5 Protein as Anti-HIV-1 Factor

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