Complete Nucleotide Sequence of the First KPC-2- and SHV-12-Encoding IncX Plasmid, pKpS90, from Klebsiella pneumoniae

Najiby Kassis-Chikhani (1, 2) , L. Frangeul (3) , Laurence Drieux (4, 5) , Christian Sengelin (1, 2) , Vincent Jarlier (4, 6) , Sylvain Brisse (7) , Guillaume Arlet (4, 8) , Dominique Decré (4, 9)

1 Hôpital Paul Brousse
2 AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP)
3 Génopole
4 Laboratoire de Bactériologie, Faculté de médecine
5 CHU Charles Foix [AP-HP]
6 CHU Pitié-Salpêtrière [AP-HP]
7 PF8 - Génotypage des Pathogènes et Santé Publique (Plate-forme)
8 Service de Bactériologie [CHU Tenon]
9 Service de microbiologie [CHU Saint-Antoine]

We report the complete nucleotide sequence of the pKpS90 plasmid, carrying the bla KPC-2 and bla SHV-12 genes. This plasmid was isolated from a sequence type 258 (ST258) Klebsiella pneumoniae strain responsible for an outbreak in a French university hospital in 2009. pKpS90 is a 53,286-bp plasmid that belongs to the IncX incompatibility group. pKpS90 consists of a backbone from IncX plasmids, in which the KPC-2-encoding Tn 4401 transposon and a bla SHV-12 -encoding region have been inserted.

Domains: Computer Science [cs]; Bioinformatics [q-bio.QM]; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Genomics [q-bio.GN]; Life Sciences [q-bio]; Microbiology and Parasitology; Virology

Najiby Kassis-Chikhani, L. Frangeul, Laurence Drieux, Christian Sengelin, Vincent Jarlier, et al.. Complete Nucleotide Sequence of the First KPC-2- and SHV-12-Encoding IncX Plasmid, pKpS90, from Klebsiella pneumoniae. Antimicrobial Agents and Chemotherapy, 2012, 57 (1), pp.618-620. ⟨10.1128/AAC.01712-12⟩. ⟨pasteur-03252025⟩