Geographic distribution of anti-Leptospira antibodies in humans in Côte d’Ivoire, West Africa
Résumé
The study was carry out on stored human specimens previously collected for the national surveillance 39 system for communicable diseases in 2014. Only negative specimens were included in the study. 40 One to 13 serum samples were randomly selected per health district based on the number of notified 41 cases by health districts in the year to obtain a sample size of 384. 42 Blood samples were collected from each patient who matches the clinical WHO's case definition for 43 notifiable disease. Samples were sent, at +4°C, to the National Reference Centre at the Institut Pasteur 44 de Côte d'Ivoire for analysis. Anti-Leptospira antibody testing was carried out at Institut Pasteur de 45 Côte d'Ivoire (IPCI) and Institut Pasteur in Paris (IP). We used as screening methods the ELISA 46 against type IgG anti-Leptospira antibodies previously describe by Bourhy et al. (2013) in IPCI [7]. 47 Confirmation was carried out by MAT in IP with a threshold of 1:50. As antigens, 16 serogroups were 48 used (table 1). 49 Socio-demographic and clinical data from the National Reference Centre, which manage the samples 50 and results for analysis performed on studied samples, were used to build a database using Epi Info 51 software (CDC, Atlanta, Georgia). Data analyses were done using R software 3.2.0 [8].
Domaines
Sciences du Vivant [q-bio]
Origine : Fichiers produits par l'(les) auteur(s)