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Journal articles
Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system
Lenka Horváthová
1
Vojtěch Žárský
1
Tomáš Pánek
2
Romain Derelle
3
Jan Pyrih
4, 5
Alžběta Motyčková
1
Veronika Klápšťová
1
Martina Vinopalová
1
Lenka Marková
6, 1
Luboš Voleman
1
Vladimír Klimeš
2
Markéta Petrů
1
Zuzana Vaitová
1
Ivan Čepička
7
Klára Hryzáková
7
Karel Harant
7
Michael Gray
8
Mohamed Chami
9
Ingrid Guilvout
10
Olivera Francetic
10
B. Franz Lang
11
Čestmír Vlček
5
Anastasios Tsaousis
4
Marek Eliáš
2, *
Pavel Doležal
1, *
*
Corresponding author
Jan Pyrih 4, 5
Author
Alžběta Motyčková 1
Author
Veronika Klápšťová 1
Author
Martina Vinopalová 1
Author
Michael W Gray 8
Author
Mohamed Chami 9
Author
Ingrid Guilvout 10
Author
Olivera Francetic 10
Author IdHAL : olivera-francetic ORCID : https://orcid.org/0000-0002-4145-5314
B. Franz Lang 11
Author
Čestmír Vlček 5
Author
Anastasios D Tsaousis 4
Author
Marek Eliáš 2
Correspondent author
Pavel Doležal 1
Correspondent author
1
Dpt of Parasitology [Prague] (Czech Republic)
- CU - Charles University [Prague] (Ovocný trh 3-5, Prague 1, 116 36 Czech Republic - Czech Republic)
2
Ostravská univerzita / University of Ostrava (Dvořákova 7
701 03 Ostrava - Czech Republic)
3
University of Birmingham [Birmingham] (Edgbaston
Birmingham B15 2TT - United Kingdom)
4
University of Kent [Canterbury] (Canterbury,
Kent,
CT2 7NZ - United Kingdom)
5
CAS - Czech Academy of Sciences [Prague] (Národní 3, 117 20 Prague 1 - Czech Republic)
6
MED / MUNI - Faculty of Medicine [Brno] (Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic - Czech Republic)
- MUNI - Masaryk University [Brno] (Kamenice 5, Brno CZ-62500, Czech Republic - Czech Republic)
7
CU - Charles University [Prague] (Ovocný trh 3-5, Prague 1, 116 36 Czech Republic - Czech Republic)
8
Dalhousie University [Halifax] ( 6299 South St, Halifax, NS B3H 4R2 - Canada)
9
Center for Cellular Imaging and Nanoanalytics (Biozentrum, CH-4056 Basel, Switzerland - Switzerland)
- Unibas - University of Basel (Petersplatz 1, P. O. Box 4001 Basel - Switzerland)
10
Biochimie des Interactions Macromoléculaires / Biochemistry of Macromolecular Interactions (Département de Biologie structurale et Chimie, 25-28 rue du Docteur Roux 75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR3528 (France)
11
UdeM - Université de Montréal (2900 Boulevard Edouard-Montpetit, Montréal, QC H3T 1J4 - Canada)
Abstract : The type 2 secretion system (T2SS) is present in some Gram-negative eubacteria and used to secrete proteins across the outer membrane. Here we report that certain representative heteroloboseans, jakobids, malawimonads and hemimastigotes unexpectedly possess homologues of core T2SS components. We show that at least some of them are present in mitochondria, and their behaviour in biochemical assays is consistent with the presence of a mitochondrial T2SS-derived system (miT2SS). We additionally identified 23 protein families co-occurring with miT2SS in eukaryotes. Seven of these proteins could be directly linked to the core miT2SS by functional data and/or sequence features, whereas others may represent different parts of a broader functional pathway, possibly also involving the peroxisome. Its distribution in eukaryotes and phylogenetic evidence together indicate that the miT2SScentred pathway is an ancestral eukaryotic trait. Our findings thus have direct implications for the functional properties of the early mitochondrion.
Document type :
Journal articles
Complete list of metadata
https://hal-pasteur.archives-ouvertes.fr/pasteur-03247185
Contributor : Olivera Francetic Connect in order to contact the contributor
Submitted on : Wednesday, June 2, 2021 - 8:49:58 PM
Last modification on : Thursday, April 7, 2022 - 10:10:45 AM
Long-term archiving on: : Friday, September 3, 2021 - 8:06:42 PM
Contributor : Olivera Francetic Connect in order to contact the contributor
Submitted on : Wednesday, June 2, 2021 - 8:49:58 PM
Last modification on : Thursday, April 7, 2022 - 10:10:45 AM
Long-term archiving on: : Friday, September 3, 2021 - 8:06:42 PM
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41467_2021_Article_23046.pdf
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Distributed under a Creative Commons Attribution 4.0 International License
Identifiers
- HAL Id : pasteur-03247185, version 1
- DOI : 10.1038/s41467-021-23046-7
- PUBMED : 34011950
- PUBMEDCENTRAL : PMC8134430
Citation
Lenka Horváthová, Vojtěch Žárský, Tomáš Pánek, Romain Derelle, Jan Pyrih, et al.. Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system. Nature Communications, Nature Publishing Group, 2021, 12 (1), pp.2947. ⟨10.1038/s41467-021-23046-7⟩. ⟨pasteur-03247185⟩
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