Using ProteoCombiner to integrate bottom-up and top-down proteomics data to improve proteoform identification

Diogo Borges Lima; Mathieu Dupré; Magalie Duchateau; Quentin Giai Gianetto; Martial Rey; Mariette Matondo; Julia Chamot-Rooke

doi:10.21203/rs.2.10327/v1

Preprints, Working Papers, ...

Using ProteoCombiner to integrate bottom-up and top-down proteomics data to improve proteoform identification

Diogo Borges Lima ^{1, *} Mathieu Dupré ¹ Magalie Duchateau ² Quentin Giai Gianetto ^{2, 3} Martial Rey ¹ Mariette Matondo ² Julia Chamot-Rooke ^{1, *}

* Corresponding author

1 UTechS MSBio - Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology

2 Plateforme de Protéomique / Proteomics platform

UTechS MSBio - Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology

3 Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB

Abstract : Here we present a high-performance software for proteome analysis that combines different mass spectrometric approaches, such as, top-down for intact protein analyses and bottom-up, for proteolytic fragment characterization. ProteoCombiner capitalizes on the data arising from different experiments and proteomics search engines and presents the results in a user-friendly manner. Our tool also provides a rapid and easy visualization, manual validation and comparison of the identified proteoform sequences, including post-translational modifications (PTM) characterization. Thus, ProteoCombiner is recommended for studies dealing with different proteomics strategies, in order to increase confidence in proteoform identification including PTMs.

Keywords : proteomics bioinformatics top-down bottom-up mass spectrometry

Document type :

Preprints, Working Papers, ...

Domain :

Computer Science [cs] / Bioinformatics [q-bio.QM]

Life Sciences [q-bio] / Biochemistry, Molecular Biology / Genomics [q-bio.GN]

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https://hal-pasteur.archives-ouvertes.fr/pasteur-03243560
Contributor : Quentin Giai Gianetto <>
Submitted on : Monday, May 31, 2021 - 4:38:43 PM
Last modification on : Wednesday, June 23, 2021 - 2:18:12 PM

Using ProteoCombiner to integrate bottom-up and top-down proteomics data to improve proteoform identification

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Using ProteoCombiner to integrate bottom-up and top-down proteomics data to improve proteoform identification

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