Using ProteoCombiner to integrate bottom-up and top-down proteomics data to improve proteoform identification - Archive ouverte HAL Access content directly
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Using ProteoCombiner to integrate bottom-up and top-down proteomics data to improve proteoform identification

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Abstract

Here we present a high-performance software for proteome analysis that combines different mass spectrometric approaches, such as, top-down for intact protein analyses and bottom-up, for proteolytic fragment characterization. ProteoCombiner capitalizes on the data arising from different experiments and proteomics search engines and presents the results in a user-friendly manner. Our tool also provides a rapid and easy visualization, manual validation and comparison of the identified proteoform sequences, including post-translational modifications (PTM) characterization. Thus, ProteoCombiner is recommended for studies dealing with different proteomics strategies, in order to increase confidence in proteoform identification including PTMs.
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Dates and versions

pasteur-03243560 , version 1 (05-11-2021)

Licence

Attribution - CC BY 4.0

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Diogo Borges Lima, Mathieu Dupré, Magalie Duchateau, Quentin Giai Gianetto, Martial Jean-Pierre Rey, et al.. Using ProteoCombiner to integrate bottom-up and top-down proteomics data to improve proteoform identification. 2021. ⟨pasteur-03243560⟩
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