Skip to Main content Skip to Navigation
Journal articles

Ligand Binding but Undetected Functional Response of FcR after Their Capture by T Cells via Trogocytosis

Abstract : Intercellular transfer of cell surface proteins by trogocytosis is common and could affect T cell responses. Yet, the role of trogocytosis in T cell function is still elusive, and it is unknown whether a molecule, once captured by T cells, harbors the same biological properties as in donor APC. In this study, we showed that FcgammaR as well as the associated FcRgamma subunit could be detected at high levels on murine and human T cells after their intercellular transfer from FcgammaR-expressing APC. Capture of FcgammaR occurred during coculture of T cells with FcgammaR-expressing APC upon Ab- or Ag-mediated T cell stimulation. Once captured by T cells, FcgammaR were expressed in a conformation compatible with physiological function and conferred upon T cells the ability to bind immune complexes and to provision B cells with this source of Ag. However, we were unable to detect downstream signal or signaling-dependent function following the stimulation of FcgammaR captured by T cells, and biochemical studies suggested the improper integration of FcgammaR in the recipient T cell membrane. Thus, our study demonstrates that T cells capture FcgammaR that can efficiently exert ligand-binding activity, which, per se, could have functional consequences in T cell-B cell cooperation.
Document type :
Journal articles
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03242928
Contributor : Pierre Bruhns <>
Submitted on : Monday, May 31, 2021 - 12:37:19 PM
Last modification on : Wednesday, June 30, 2021 - 3:35:10 AM

Links full text

Identifiers

Collections

Citation

Denis Hudrisier, Béatrice Clemenceau, Stéphanie Balor, Sandrine Daubeuf, Eddy Magdeleine, et al.. Ligand Binding but Undetected Functional Response of FcR after Their Capture by T Cells via Trogocytosis. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2009, 183 (10), pp.6102-6113. ⟨10.4049/jimmunol.0900821⟩. ⟨pasteur-03242928⟩

Share

Metrics

Record views

14