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Real-time tracking of bacterial membrane vesicles reveals enhanced membrane traffic upon antibiotic exposure

Abstract : Membrane vesicles are ubiquitous carriers of molecular information. A broad understanding of the biological functions of membrane vesicles in bacteria remains elusive because of the imaging challenges during real-time in vivo experiments. Here, we provide a quantitative analysis of the motion of individual vesicles in living microbes using fluorescence microscopy, and we show that while vesicle free diffusion in the intercellular space is rare, vesicles mostly disperse along the bacterial surfaces. Most remarkably, when bacteria are challenged with low doses of antibiotics, vesicle production and traffic, quantified by instantaneous vesicle speeds and total traveled distance per unit time, are significantly enhanced. Furthermore, the enhanced vesicle movement is independent of cell clustering properties but rather is associated with a reduction of the density of surface appendages in response to antibiotics. Together, our results provide insights into the emerging field of spatial organization and dynamics of membrane vesicles in microcolonies.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03242762
Contributor : Julia Bos <>
Submitted on : Monday, May 31, 2021 - 11:36:21 AM
Last modification on : Monday, June 14, 2021 - 10:06:01 PM

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Distributed under a Creative Commons Attribution 4.0 International License

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Julia Bos, Luis Cisneros, Didier Mazel. Real-time tracking of bacterial membrane vesicles reveals enhanced membrane traffic upon antibiotic exposure. Science Advances , American Association for the Advancement of Science (AAAS), 2021, 7 (4), pp.eabd1033. ⟨10.1126/sciadv.abd1033⟩. ⟨pasteur-03242762⟩

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