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Detection of Hepatitis E Virus RNA in Stools and Serum by Reverse Transcription-PCR

Abstract : Stools and sera collected during an experimental hepatitis E virus (HEV) infection in monkeys and collected from humans with acute HEV infections during epidemic and sporadic cases were analyzed by reverse transcription-PCR. Two methods for RNA purification were compared. Proteinase K digestion and phenolchloroform extraction were more efficient than guanidinium isothiocyanate extraction in improving the sensitivity and specificity for the detection of HEV genomes.
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Submitted on : Wednesday, May 26, 2021 - 11:07:42 AM
Last modification on : Thursday, April 7, 2022 - 10:10:54 AM

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Salih Turkoglu, Yamina Lazizi, Jihong Meng, Aphroditis Kordosi, Pascal Dubreuil, et al.. Detection of Hepatitis E Virus RNA in Stools and Serum by Reverse Transcription-PCR. Journal of Clinical Microbiology, American Society for Microbiology, 1996, 34 (6), pp.1568-1571. ⟨10.1128/jcm.34.6.1568-1571.1996⟩. ⟨pasteur-03236330⟩

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