Detection of Hepatitis E Virus RNA in Stools and Serum by Reverse Transcription-PCR

Salih Turkoglu (1) , Yamina Lazizi (1) , Jihong Meng (2) , Aphroditis Kordosi (2) , Pascal Dubreuil (1) , Bernadette Crescenzo (2) , Soumya Benjelloun (3) , Patrice Nordmann (1) , Jacques Pillot (1, 2)

1 AP-HP - Hôpital Antoine Béclère [Clamart]
2 Immunologie microbienne
3 Institut Pasteur du Maroc

Stools and sera collected during an experimental hepatitis E virus (HEV) infection in monkeys and collected from humans with acute HEV infections during epidemic and sporadic cases were analyzed by reverse transcription-PCR. Two methods for RNA purification were compared. Proteinase K digestion and phenolchloroform extraction were more efficient than guanidinium isothiocyanate extraction in improving the sensitivity and specificity for the detection of HEV genomes.

Domaines: Sciences du Vivant [q-bio]

Salih Turkoglu, Yamina Lazizi, Jihong Meng, Aphroditis Kordosi, Pascal Dubreuil, et al.. Detection of Hepatitis E Virus RNA in Stools and Serum by Reverse Transcription-PCR. Journal of Clinical Microbiology, 1996, 34 (6), pp.1568-1571. ⟨10.1128/jcm.34.6.1568-1571.1996⟩. ⟨pasteur-03236330⟩