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Identification of a Strong Enhancer Element Upstream from the Pregenomic RNA Start Site of the Duck Hepatitis B Virus Genome

Abstract : The genome of the duck hepatitis B virus (DHBV) contains an enhancer element. This sequence, of 192 bp, is located in the 3'-terminal coding region of the DNA polymerase gene (nucleotides 2159 to 2351), upstream from the pregenomic RNA start site. This enhancer potentiates a marked increased activity from the heterologous thymidine kinase promoter in an orientation-independent manner and at a proximal, as well as a distal, location. The DHBV enhancer activates transcription in a relatively cell-type-independent manner. Sequence homologies with the nuclear factor EF-C binding site are located in the DHBV enhancer. By using the HepG2 nuclear extracts and the DHBV enhancer as probes, a complex was observed in mobility shift assays.
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Contributor : Bernadette Crescenzo Connect in order to contact the contributor
Submitted on : Wednesday, May 26, 2021 - 10:59:38 AM
Last modification on : Tuesday, October 19, 2021 - 10:29:31 PM

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B. Crescenzo-Chaigne, J. Pillot, A. Lilienbaum, M. Levrero, E. Elfassi. Identification of a Strong Enhancer Element Upstream from the Pregenomic RNA Start Site of the Duck Hepatitis B Virus Genome. Journal of Virology, American Society for Microbiology, 1991, 65 (7), pp.3882-3886. ⟨10.1128/JVI.65.7.3882-3886.1991⟩. ⟨pasteur-03236273⟩

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