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Journal articles
Ubc9 Is Required for Positive Selection and Late-Stage Maturation of Thymocytes
Abstract : SUMOylation is an important posttranslational modification that regulates protein function in diverse biological processes. However, its role in early T cell development has not been genetically studied. UBC9 is the only E2 enzyme for all SUMOylation. In this study, by selectively deleting Ubc9 gene in T cells, we have investigated the functional roles of SUMOylation in T cell development. Loss of Ubc9 results in a significant reduction of CD4 and CD8 single-positive lymphocytes in both thymus and periphery. Ubc9-deficient cells exhibit defective late-stage maturation post the initial positive selection with increased apoptosis and impaired proliferation, among which attenuated IL-7 signaling was correlated with the decreased survival of Ubc9-deficent CD8 single-positive cells. Furthermore, NFAT nuclear retention induced by TCR signals was regulated by SUMOylation during thymocytes development. Our study thus reveals a novel posttranslational mechanism underlying T cell development.
https://hal-pasteur.archives-ouvertes.fr/pasteur-03234899
Contributor : Samia Cheriet Rauline Connect in order to contact the contributor
Submitted on : Tuesday, May 25, 2021 - 3:30:38 PM
Last modification on : Thursday, April 7, 2022 - 10:10:29 AM
Contributor : Samia Cheriet Rauline Connect in order to contact the contributor
Submitted on : Tuesday, May 25, 2021 - 3:30:38 PM
Last modification on : Thursday, April 7, 2022 - 10:10:29 AM
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