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Ubc9 Is Required for Positive Selection and Late-Stage Maturation of Thymocytes

Abstract : SUMOylation is an important posttranslational modification that regulates protein function in diverse biological processes. However, its role in early T cell development has not been genetically studied. UBC9 is the only E2 enzyme for all SUMOylation. In this study, by selectively deleting Ubc9 gene in T cells, we have investigated the functional roles of SUMOylation in T cell development. Loss of Ubc9 results in a significant reduction of CD4 and CD8 single-positive lymphocytes in both thymus and periphery. Ubc9-deficient cells exhibit defective late-stage maturation post the initial positive selection with increased apoptosis and impaired proliferation, among which attenuated IL-7 signaling was correlated with the decreased survival of Ubc9-deficent CD8 single-positive cells. Furthermore, NFAT nuclear retention induced by TCR signals was regulated by SUMOylation during thymocytes development. Our study thus reveals a novel posttranslational mechanism underlying T cell development.
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Submitted on : Tuesday, May 25, 2021 - 3:30:38 PM
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Aibo Wang, Xiao Ding, Maud Demarque, Xindong Liu, Deng Pan, et al.. Ubc9 Is Required for Positive Selection and Late-Stage Maturation of Thymocytes. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2017, 198 (9), pp.3461-3470. ⟨10.4049/jimmunol.1600980⟩. ⟨pasteur-03234899⟩



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