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Journal Articles Journal of Immunology Year : 2017

Ubc9 Is Required for Positive Selection and Late-Stage Maturation of Thymocytes

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Abstract

SUMOylation is an important posttranslational modification that regulates protein function in diverse biological processes. However, its role in early T cell development has not been genetically studied. UBC9 is the only E2 enzyme for all SUMOylation. In this study, by selectively deleting Ubc9 gene in T cells, we have investigated the functional roles of SUMOylation in T cell development. Loss of Ubc9 results in a significant reduction of CD4 and CD8 single-positive lymphocytes in both thymus and periphery. Ubc9-deficient cells exhibit defective late-stage maturation post the initial positive selection with increased apoptosis and impaired proliferation, among which attenuated IL-7 signaling was correlated with the decreased survival of Ubc9-deficent CD8 single-positive cells. Furthermore, NFAT nuclear retention induced by TCR signals was regulated by SUMOylation during thymocytes development. Our study thus reveals a novel posttranslational mechanism underlying T cell development.

Dates and versions

pasteur-03234899 , version 1 (25-05-2021)

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Aibo Wang, Xiao Ding, Maud Demarque, Xindong Liu, Deng Pan, et al.. Ubc9 Is Required for Positive Selection and Late-Stage Maturation of Thymocytes. Journal of Immunology, 2017, 198 (9), pp.3461-3470. ⟨10.4049/jimmunol.1600980⟩. ⟨pasteur-03234899⟩

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