 |
Journal articles
Noncanonical DNA polymerization by aminoadenine-based siphoviruses
Valerie Pezo
1
Faten Jaziri
1
Pierre-Yves Bourguignon
2, 3
Dominique Louis
4
Deborah Jacobs-Sera
5
Jef Rozenski
6
Sylvie Pochet
7
Piet Herdewijn
6
Graham Hatfull
5
Pierre-Alexandre Kaminski
8
Philippe Marliere
1, 3, *
*
Corresponding author
Valerie Pezo 1
Author
Faten Jaziri 1
Author
Pierre-Yves Bourguignon 2, 3
Author
Dominique Louis 4
Author
Graham Hatfull 5
Author
Pierre-Alexandre Kaminski 8
Author
Philippe Marliere 1, 3
Correspondent author
1
UMR 8030 - Génomique métabolique (GENOSCOPE, 2 rue Gaston Crémieux 91057 Evry Cedex - France)
- GENOSCOPE - Genoscope - Centre national de séquençage [Evry] : DRF/JACOB (2, rue Gaston Crémieux CP5706 91057 EVRY Cedex - France)
- Université Paris-Saclay (Bâtiment Bréguet, 3 Rue Joliot Curie 2e ét, 91190 Gif-sur-Yvette - France)
- DRF (CEA) - Direction de Recherche Fondamentale (CEA) : DRF/JACOB (Direction de Recherche Fondamentale
Fundamental Research Division
CEA Saclay
91191 Gif-sur-Yvette
- France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- CNRS - Centre National de la Recherche Scientifique : UMR8030 (France)
- UEVE - Université d'Évry-Val-d'Essonne (Boulevard François Mitterrand 91025 Evry Cedex - France)
2
Werkstatt fuer Potenzielle Genetik [Berlin] (Germany)
3
TESSI [Paris] (81 Rue Réaumur, 75002 Paris - France)
4
Alderys [Orsay] (86 Rue de Paris, 91400 Orsay - France)
5
PITT - University of Pittsburgh (4200 Fifth Avenue Pittsburgh, PA 15260 - United States)
- Pennsylvania Commonwealth System of Higher Education (PCSHE) (United States)
6
Rega Institute for Medical Research [Leuven, België] (Minderbroedersstraat 10, B 3000 Leuven - Belgium)
- KU Leuven - Catholic University of Leuven - Katholieke Universiteit Leuven (Oude Markt 13 - bus 5005, 3000 Leuven - Belgium)
7
CNR - UMR3523 - Chimie organique (28, rue du Docteur Roux 75724 Paris Cedex 15 - France)
- INC - Institut de Chimie du CNRS (3 Rue Michel-Ange 75 016 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR3523 (France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
8
Biologie des Bactéries pathogènes à Gram-positif (Département Microbiologie - 25-28 rue du Dr Roux 75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : ERL3526 (France)
Abstract : Bacteriophage genomes harbor the broadest chemical diversity of nucleobases across all life forms. Certain DNA viruses that infect hosts as diverse as cyanobacteria, proteobacteria, and actinobacteria exhibit wholesale substitution of aminoadenine for adenine, thereby forming three hydrogen bonds with thymine and violating Watson-Crick pairing rules. Aminoadenine-encoded DNA polymerases, homologous to the Klenow fragment of bacterial DNA polymerase I that includes 3′-exonuclease but lacks 5′-exonuclease, were found to preferentially select for aminoadenine instead of adenine in deoxynucleoside triphosphate incorporation templated by thymine. Polymerase genes occur in synteny with genes for a biosynthesis enzyme that produces aminoadenine deoxynucleotides in a wide array of Siphoviridae bacteriophages. Congruent phylogenetic clustering of the polymerases and biosynthesis enzymes suggests that aminoadenine has propagated in DNA alongside adenine since archaic stages of evolution.
Document type :
Journal articles
Complete list of metadata
https://hal-pasteur.archives-ouvertes.fr/pasteur-03228903
Contributor : Pierre Alexandre Kaminski Connect in order to contact the contributor
Submitted on : Tuesday, May 18, 2021 - 2:49:14 PM
Last modification on : Tuesday, January 25, 2022 - 3:31:21 AM
Contributor : Pierre Alexandre Kaminski Connect in order to contact the contributor
Submitted on : Tuesday, May 18, 2021 - 2:49:14 PM
Last modification on : Tuesday, January 25, 2022 - 3:31:21 AM
Links full text
Identifiers
- HAL Id : pasteur-03228903, version 1
- DOI : 10.1126/science.abe6542
- PUBMED : 33926956
Collections
PASTEUR | CEA | INC-CNRS | JACOB | CEA-DRF | UNIV-EVRY | GENOSCOPE | CNRS | ANR | CEA-UPSAY | GS-BIOSPHERA | GENOMIQUE-METABOLIQUE | GS-LIFE-SCIENCES-HEALTH
Citation
Valerie Pezo, Faten Jaziri, Pierre-Yves Bourguignon, Dominique Louis, Deborah Jacobs-Sera, et al.. Noncanonical DNA polymerization by aminoadenine-based siphoviruses. Science, American Association for the Advancement of Science, 2021, 372 (6541), pp.520-524. ⟨10.1126/science.abe6542⟩. ⟨pasteur-03228903⟩
Metrics
Les métriques sont temporairement indisponibles