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A third purine biosynthetic pathway encoded by aminoadenine-based viral DNA genomes

Abstract : Cells have two purine pathways that synthesize adenine and guanine ribonucleotides from phosphoribose via inosylate. A chemical hybrid between adenine and guanine, 2-aminoadenine (Z), replaces adenine in the DNA of the cyanobacterial virus S-2L. We show that S-2L and Vibrio phage PhiVC8 encode a third purine pathway catalyzed by PurZ, a distant paralog of succinoadenylate synthase (PurA), the enzyme condensing aspartate and inosylate in the adenine pathway. PurZ condenses aspartate with deoxyguanylate into dSMP (N6-succino-2-amino-2′-deoxyadenylate), which undergoes defumarylation and phosphorylation to give dZTP (2-amino-2′-deoxyadenosine-5′-triphosphate), a substrate for the phage DNA polymerase. Crystallography and phylogenetics analyses indicate a close relationship between phage PurZ and archaeal PurA enzymes. Our work elucidates the biocatalytic innovation that remodeled a DNA building block beyond canonical molecular biology.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03228896
Contributor : Pierre Alexandre Kaminski Connect in order to contact the contributor
Submitted on : Tuesday, May 18, 2021 - 2:47:14 PM
Last modification on : Tuesday, October 19, 2021 - 10:29:30 PM

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Dona Sleiman, Pierre Simon Garcia, Marion Lagune, Jerome Loc'H, Ahmed Haouz, et al.. A third purine biosynthetic pathway encoded by aminoadenine-based viral DNA genomes. Science, American Association for the Advancement of Science, 2021, 372 (6541), pp.516-520. ⟨10.1126/science.abe6494⟩. ⟨pasteur-03228896⟩

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