Skip to Main content Skip to Navigation
New interface
Journal articles

Genomic determinants for initiation and length of natural antisense transcripts in Entamoeba histolytica

Abstract : Natural antisense transcripts (NAT) have been reported in prokaryotes and eukaryotes. While the functions of most reported NATs remain unknown, their potentials in regulating the transcription of their counterparts have been speculated. Entamoeba histolytica, which is a unicellular eukaryotic parasite, has a compact protein-coding genome with very short intronic and intergenic regions. The regulatory mechanisms of gene expression in this compact genome are under-described. In this study, by genome-wide mapping of RNA-Seq data in the genome of E. histolytica, we show that a substantial fraction of its protein-coding genes (28%) has significant transcription on their opposite strand (i.e. NAT). Intriguingly, we found the location of transcription start sites or polyadenylation sites of NAT are determined by the specific motifs encoded on the opposite strand of the gene coding sequences, thereby providing a compact regulatory system for gene transcription. Moreover, we demonstrated that NATs are globally up-regulated under various environmental conditions including temperature stress and pathogenicity. While NATs do not appear to be consequences of spurious transcription, they may play a role in regulating gene expression in E. histolytica, a hypothesis which needs to be tested.
Document type :
Journal articles
Complete list of metadata
Contributor : jean-yves coppee Connect in order to contact the contributor
Submitted on : Tuesday, May 18, 2021 - 11:30:57 AM
Last modification on : Thursday, September 1, 2022 - 11:14:45 AM


Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License




Damien Mornico, Chung-Chau Hon, Mikael Koutero, Christian Weber, Jean-Yves Coppee, et al.. Genomic determinants for initiation and length of natural antisense transcripts in Entamoeba histolytica. Scientific Reports, 2020, 10 (1), pp.20190. ⟨10.1038/s41598-020-77010-4⟩. ⟨pasteur-03228542⟩



Record views


Files downloads